FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
货号： PCK005
Price： ￥5080 ￥2180 ￥1180 ￥580 ￥7480
产品信息
| 产品形态 | 液体 |
| 产品规格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 别名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 物种 | 人 |
| 表达宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 检索号 | BAG70135.1 |
| 分子量 | 17.2kDa |
| 表观分子量 | 17kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.75 ng/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6个月 |
| 制剂 | Supplied as a 0.2 μm filtered solution of 20 mM Citrate, 10% Trehalose, 150 mM NaCl, 0.04% PS80, 0.5 mM EDTA, pH 5.5. |
| 背景介绍 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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Elevation of LEM Domain Containing 1 Predicts Poor Prognosis of NSCLC Patients and Triggers Malignant Stemness and Invasion of NSCLC Cells by Stimulating PI3K/AKT Pathway(2023)
期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影响因子：2.500
引用产品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer(2022)
期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子：5.600
引用产品： PC-9 细胞, A549 [A-549] 细胞, NCI-H1299 细胞, NCI-H1975 细胞, BEAS-2B 细胞, RPMI-1640 培养基, BEAS-2B细胞专用培养基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:这个重组蛋白如果我想加入细胞里，用哪个溶解呢 ？因为随产品附赠了一管pbs和一管无菌水
首次复融使用无菌水，复融后如果还需要进一步稀释的话，使用含海藻糖的PBS进行稀释。
识别码示意图
