货号: CL-0021
Price: ¥1600 ¥1600
生长培养基: MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | ACHN(人肾细胞腺癌细胞) |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 人干扰素可抑制ACHN细胞生长;ACHN细胞能用于人干扰素、干扰素诱导因子的抗肿瘤活性研究。 |
年龄(性别) | 22Y Male |
组织来源 | 肾细胞腺癌,胸水转移灶 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10<sup>7</sup> cells). |
STR位点信息
STR鉴定图
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HIF-2α/LPCAT1 orchestrates the reprogramming of lipid metabolism in ccRCC by modulating the FBXW7-mediated ubiquitination of ACLY(2025)
作者:Mintian Fei, Yi Zhang, Haolin Li, Qili Xu, Yu Gao, Cheng Yang, Weiyi Li, Chaozhao Liang, Baojun Wang, Haibing Xiao
期刊:International Journal of Biological Sciences
影响因子:8.2
引用产品: 786-O [786-0] 细胞, OS-RC-2 细胞, A-498 细胞, ACHN 细胞, Caki-1 细胞, HK-2 细胞, 293T [HEK-293T] 细胞
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Hypoxia-triggered ERRα acetylation enhanced its oncogenic role and promoted progression of renal cell carcinoma by coordinating autophagosome-lysosome fusion(2025)
作者:Feng Chun, Kong Demin, Tong Binghua, Liang Yonghui, Xu Fuyi, Yang Yangyang, Wu Yingying, Chi Xiaodong, Wei Pengfei, Yang Yang, Zhang Guilong, Tian Geng, Xu Zhaowei
期刊:Cell Death & Disease
DOI:10.1038/s41419-025-07345-1
影响因子:8.1
引用产品: HK-2 细胞, ACHN 细胞, A-498 细胞, 769-P 细胞, 786-O [786-0] 细胞, OS-RC-2 细胞, 293T [HEK-293T] 细胞
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MYBL2 promotes proliferation of clear cell renal cell carcinoma by regulating TOP2A and activating AKT/mTOR signaling pathway(2025)
作者:Yawei Huang, Xiaoqing Xi, Zhenfeng Ye, Chiyu Zhang, Yi Jiang, Fanfan Yu, Gaomin Huang
期刊:FASEB JOURNAL
影响因子:4.4
引用产品: ACHN 细胞, 特级胎牛血清
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HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC(2024)
作者:Kang Qian, Wei Li, Shangqing Ren, Weilin Peng, Bei Qing, Xinlin Liu, Xiong Wei, Liang Zhu, Yapeng Wang, Xin Jin
期刊:Advanced Science
影响因子:14.3
引用产品: A-498 细胞, 786-O [786-0] 细胞, ACHN 细胞
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TRIM65 deficiency alleviates renal fibrosis through NUDT21-mediated alternative polyadenylation(2024)
作者:Wei Sisi, Huang Xuan, Zhu Qing, Chen Tao, Zhang Yan, Tian Juan, Pan Tingyu, Zhang Lv, Xie Tao, Zhang Qi, Kuang Xian, Lei Enjun, Li Yong
期刊:CELL DEATH AND DIFFERENTIATION
DOI:10.1038/s41418-024-01336-z
影响因子:13.7
引用产品: RPMI-1640 培养基, ACHN 细胞
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TRIM65 promotes renal cell carcinoma through ubiquitination and degradation of BTG3(2024)
作者:Zhang Qi, Li Yong, Zhu Qing, Xie Tao, Xiao Yue, Zhang Feng, Li Na, Deng Keyu, Xin Hongbo, Huang Xuan
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06741-3
影响因子:9.0
引用产品: DMEM高糖 培养基, McCoy's 5A 培养基, MEM 培养基, ACHN 细胞, Caki-2 细胞, RPMI-1640 培养基, Caki-1 细胞, 769-P 细胞
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CLIP170 enhancing FOSL1 expression via attenuating ubiquitin-mediated degradation of β-catenin drives renal cell carcinoma progression(2024)
作者:Huang Yuanbin, Wen Zhihao, Tao Shuyao, Yu Zhenlong, Wang Xiaogang, Li Xiancheng, Gao Lu
期刊:CELLULAR AND MOLECULAR LIFE SCIENCES
DOI:10.1007/s00018-024-05504-9
影响因子:6.2
引用产品: 786-O [786-0] 细胞, Caki-1 细胞, 769-P 细胞, HK-2 细胞, ACHN 细胞
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Selective expansion of renal cancer stem cells using microfluidic single-cell culture arrays for anticancer drug testing(2024)
作者:Xiaogang Wang, Tao He, Zihe Chen, Jueming Chen, Yanzhang Luo, Dongguo Lin, Xiancheng Li, Dayu Liu
期刊:LAB ON A CHIP
影响因子:6.1
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, ACHN 细胞
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Identification of molecular subtypes and diagnostic model in clear cell renal cell carcinoma based on collagen-related genes may predict the response of immunotherapy(2024)
作者:Hong Yulong, Lv Zhengtong, Xing Zhuo, Xu Haozhe, Chand Harripersaud, Li Yuan
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1325447
影响因子:5.6
引用产品: 786-O [786-0] 细胞, ACHN 细胞
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Disulfideptosis-associated lncRNAs reveal features of prognostic, immune escape, tumor mutation, and tumor malignant progression in renal clear cell carcinoma(2024)
作者:Xungang Li, Xinxi Deng, Taobin Liu, Wensheng Zhang, Jin Tao
期刊:Aging-US
影响因子:5.2
引用产品: 786-O [786-0] 细胞, OS-RC-2 细胞, A-498 细胞, ACHN 细胞, HK-2 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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