货号: CL-0052
Price: ¥1600 ¥1600
生长培养基: McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | Caki-1(人肾透明细胞癌皮肤转移细胞) |
别称 | CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | Caki-1细胞的超微结构中包含许多微绒毛、少许微丝、许多小线粒体、充分发育的高尔基体、内质网、许多脂滴和多层体,次级溶酶体;目前,没有在Caki-1细胞内发现病毒颗粒。 |
年龄(性别) | 49Y Male |
组织来源 | 肾透明细胞癌皮肤转移 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters. |
STR位点信息
STR鉴定图
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HIF-2α/LPCAT1 orchestrates the reprogramming of lipid metabolism in ccRCC by modulating the FBXW7-mediated ubiquitination of ACLY(2025)
作者:Mintian Fei, Yi Zhang, Haolin Li, Qili Xu, Yu Gao, Cheng Yang, Weiyi Li, Chaozhao Liang, Baojun Wang, Haibing Xiao
期刊:International Journal of Biological Sciences
影响因子:8.2
引用产品: 786-O [786-0] 细胞, OS-RC-2 细胞, A-498 细胞, ACHN 细胞, Caki-1 细胞, HK-2 细胞, 293T [HEK-293T] 细胞
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Identifying ADME-related gene signature for immune landscape and prognosis in KIRC by single-cell and spatial transcriptome analysis(2025)
作者:Wang Hongyun, Li Feizhou, Wang Qiong, Guo Xinyuan, Chen Xinbing, Zou Xinrong, Yuan Jun
期刊:Scientific Reports
DOI:10.1038/s41598-024-84018-7
影响因子:3.8
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, HK-2 细胞
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TRIM65 promotes renal cell carcinoma through ubiquitination and degradation of BTG3(2024)
作者:Zhang Qi, Li Yong, Zhu Qing, Xie Tao, Xiao Yue, Zhang Feng, Li Na, Deng Keyu, Xin Hongbo, Huang Xuan
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06741-3
影响因子:9.0
引用产品: DMEM高糖 培养基, McCoy's 5A 培养基, MEM 培养基, ACHN 细胞, Caki-2 细胞, RPMI-1640 培养基, Caki-1 细胞, 769-P 细胞
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Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells(2024)
作者:Li Ziyao, Tian Ye, Zong Huafeng, Wang Xuelei, Li Dongyang, Keranmu Adili, Xin Shiyong, Ye Bowen, Bai Rong, Chen Weihua, Yang Guosheng, Ye Lin, Wang Siyan
期刊:APOPTOSIS
DOI:10.1007/s10495-024-01953-6
影响因子:7.2
引用产品: HK-2 细胞, Caki-1 细胞, 769-P 细胞, 786-O [786-0] 细胞, SW839 细胞
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CLIP170 enhancing FOSL1 expression via attenuating ubiquitin-mediated degradation of β-catenin drives renal cell carcinoma progression(2024)
作者:Huang Yuanbin, Wen Zhihao, Tao Shuyao, Yu Zhenlong, Wang Xiaogang, Li Xiancheng, Gao Lu
期刊:CELLULAR AND MOLECULAR LIFE SCIENCES
DOI:10.1007/s00018-024-05504-9
影响因子:6.2
引用产品: 786-O [786-0] 细胞, Caki-1 细胞, 769-P 细胞, HK-2 细胞, ACHN 细胞
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Selective expansion of renal cancer stem cells using microfluidic single-cell culture arrays for anticancer drug testing(2024)
作者:Xiaogang Wang, Tao He, Zihe Chen, Jueming Chen, Yanzhang Luo, Dongguo Lin, Xiancheng Li, Dayu Liu
期刊:LAB ON A CHIP
影响因子:6.1
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, ACHN 细胞
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TRIM26 inhibits clear cell renal cell carcinoma progression through destabilizing ETK and thus inactivation of AKT/mTOR signaling(2024)
作者:Di Zheng, Jinzhuo Ning, Hao Deng, Yuan Ruan, Fan Cheng
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-024-05273-w
影响因子:6.1
引用产品: Caki-1 细胞, OS-RC-2 细胞
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Disulfidptosis-associated LncRNA signature predicts prognosis and immune response in kidney renal clear cell carcinoma(2024)
作者:Xu Kangjie, Li Dongling, Ji Kangkang, Zhang Yanhua, Zhang Minglei, Zhou Hai, Hou Xuefeng, Jiang Jian, Zhang Zihang, Dai Hua, Sun Hang
期刊:Biology Direct
DOI:10.1186/s13062-024-00517-7
影响因子:5.7
引用产品: 786-O [786-0] 细胞, Caki-1 细胞, HK-2 细胞
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TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells(2024)
作者:Suli Lv, Zongbiao Zhang, Zhenyong Li, Qian Ke, Xianyun Ma, Neng Li, Xuefeng Zhao, Qingli Zou, Lidong Sun, Tanjing Song
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子:4.8
引用产品: ACHN 细胞, DMEM无糖 培养基, McCoy's 5A 培养基, McCoy's 5A无糖 培养基, MEM无糖(含NEAA) 培养基, MEM(含NEAA) 培养基, Caki-1 细胞
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Low ACADM expression predicts poor prognosis and suppressive tumor microenvironment in clear cell renal cell carcinoma(2024)
作者:Zhou Libin, Yin Min, Guo Fei, Yu Zefeng, Weng Guobin, Long Huimin
期刊:Scientific Reports
DOI:10.1038/s41598-024-59746-5
影响因子:4.6
引用产品: RPMI-1640 培养基, 786-O [786-0] 细胞, ACHN 细胞, 769-P 细胞, HK-2 细胞, Caki-1 细胞, McCoy's 5A 培养基, MEM(含NEAA) 培养基
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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