货号: CL-0052
Price: ¥1600 ¥1600
生长培养基: McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | Caki-1(人肾透明细胞癌皮肤转移细胞) |
别称 | CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | Caki-1细胞的超微结构中包含许多微绒毛、少许微丝、许多小线粒体、充分发育的高尔基体、内质网、许多脂滴和多层体,次级溶酶体;目前,没有在Caki-1细胞内发现病毒颗粒。 |
年龄(性别) | 49Y Male |
组织来源 | 肾透明细胞癌皮肤转移 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters. |
STR位点信息
STR鉴定图
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Crosstalk between GBP2 and M2 macrophage promotes the ccRCC progression(2024)
作者:Wei Zheng, Shujiang Ye, Bin Liu, Dan Liu, Ruyu Yan, Hongjuan Guo, Hongtao Yu, Xudong Hu, Huaiming Zhao, Kecheng Zhou, Guangyuan Li
期刊:CANCER SCIENCE
影响因子:4.5
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, THP-1 细胞
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Crosstalk of disulfidptosis-related subtypes identifying a prognostic signature to improve prognosis and immunotherapy responses of clear cell renal cell carcinoma patients(2024)
作者:Ren Lei, Liu Jinwen, Lin Qingyuan, He Tianyi, Huang Guankai, Wang Weifeng, Zhan Xunhao, He Yu, Huang Bin, Mao Xiaopeng
期刊:BMC GENOMICS
DOI:10.1186/s12864-024-10307-0
影响因子:4.4
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, HK-2 细胞, ACHN 细胞
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Identify CTBP1-DT as an immunological biomarker that promotes lipid synthesis and apoptosis resistance in KIRC(2024)
作者:Haolin Li, Mintian Fei, Yi Zhang, Qili Xu, Rui Feng, Jing Cao, Yan Qu, Haibing Xiao
期刊:GENE
DOI:10.1016/j.gene.2024.148403
影响因子:3.5
引用产品: HK-2 细胞, ACHN 细胞, A-498 细胞, 786-O [786-0] 细胞, OS-RC-2 细胞, Caki-1 细胞, RPMI-1640 培养基, MEM 培养基
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DLX5 Promotes Radioresistance in Renal Cell Carcinoma by Upregulating c-Myc Expression(2024)
作者:Danfei Hu, Mingyao Li, Xiaodong Chen
期刊:Frontiers in Bioscience-Landmark
影响因子:3.3
引用产品: 特级胎牛血清, McCoy's 5A 培养基, A-498 细胞, MEM(含NEAA) 培养基, 786-O [786-0] 细胞, 青霉素-链霉素溶液(双抗),100×, Caki-1 细胞, RPMI-1640 培养基, HK-2 细胞
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A Model to Predict Prognosis of Renal Cell Clear Cell Carcinoma Based on 3 Angiogenesis-related Long Non-coding RNAs(2024)
作者:Chen Guo, Zhang Tiansheng, Li Feng, Cui Chi, Huang Zhiyong, Gou Xin, Song Yajun, Li Yang
期刊:Journal of Cancer
影响因子:3.3
引用产品: HK-2 细胞, 786-O [786-0] 细胞, Caki-1 细胞, RCC-JF 细胞
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KAT7 Suppresses Tumorigenesis in Clear Cell Renal Cell Carcinoma (ccRCC) by Regulating Cell Cycle and Ferroptosis Sensitivity(2024)
作者:GuangYi Hong, Wei Chen, MaoDi Gong, YiKun Wu, GuoFeng Shu, Yu Xiao, Tao Zhang, Xu ShuXiong
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2024.114149
影响因子:3.3
引用产品: ACHN 细胞, Caki-1 细胞, 786-O [786-0] 细胞, Caki-2 细胞
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Centromere protein K enhances the activation of YAP1/TAZ signal cascade to drive the progression of clear cell renal cell carcinoma(2024)
作者:Ning Nan
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2024.117181
影响因子:3.3
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, Caki-2 细胞, HK-2 细胞, 769-P 细胞
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Exploring necrosis-associated mitochondrial gene signatures: revealing their role in prognosis and immunotherapy of renal clear cell carcinoma(2024)
作者:Wang Zhipeng, Zheng Fuchun, Wei Shiliang, Li Sheng, Xiong Situ, Zhang Lei, Wan Liangwei, Xu Songhui, Deng Jun, Liu Xiaoqiang
期刊:CLINICAL AND EXPERIMENTAL MEDICINE
DOI:10.1007/s10238-024-01426-9
影响因子:3.2
引用产品: 786-O [786-0] 细胞, OS-RC-2 细胞, ACHN 细胞, Caki-1 细胞, HK-2 细胞
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ETS homologous factor, controlled by lysine-specific demethylase 5B, suppresses clear cell renal cell carcinoma by inducing Filamin-B(2024)
作者:Fang Wang, Jiangbo Huang, Shun Zeng, Ying Pan, Hao Zhou
期刊:GENE
DOI:10.1016/j.gene.2024.148702
影响因子:2.6
引用产品: OS-RC-2 细胞, Caki-1 细胞, HK-2 细胞, THP-1 细胞
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PLAUR facilitates the progression of clear cell renal cell carcinoma by activating the PI3K/AKT/mTOR signaling pathway(2024)
作者:Tianzi Qin, Minyu Huang, Wenjuan Wei, Wei Zhou, Qianli Tang, Qun Huang, Ning Tang, Shasha Gai
期刊:PeerJ
影响因子:2.3
引用产品: Caki-1 细胞, ACHN 细胞, 769-P 细胞, 786-O [786-0] 细胞, 293 [HEK-293] 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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