货号: CL-0254
Price: ¥1800 ¥1800
生长培养基: MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A-498(人肾癌细胞) |
别称 | A498 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A-498细胞是由Aaronson·S建立,源自一位52岁患有肾癌的女性病人。 |
年龄(性别) | 52Y Female |
组织来源 | 肾脏 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice. |
STR位点信息
STR鉴定图
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WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression(2022)
作者:Y. J. Jing, L. C. Lin, L. L. Chen, Z. E. Huang, H. C. Qin, S. B. Li, Z. H. Chen
期刊:Biomed Research International
影响因子:3.2
引用产品: A-498 细胞
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Identification of a combined lncRNA prognostic signature and knockdown of lncRNA MANCR to inhibit progression of clear cell renal cell carcinoma by bioinformatics analysis(2022)
作者:Sheng Xue, Hanxu Guo, Shibo Wei, Yuan Song, Junfeng Huang, Shuo Deng, Qingwen Li, Wenyong Li
期刊:Translational Andrology and Urology
影响因子:2.5
引用产品: A-498 细胞, ACHN 细胞, SN12C 细胞, 786-O [786-0] 细胞, 769-P 细胞
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RRM2 Regulates Sensitivity to Sunitinib and PD-1 Blockade in Renal Cancer by Stabilizing ANXA1 and Activating the AKT Pathway(2021)
作者:Wei Xiong, Bin Zhang, Haixin Yu
期刊:Advanced Science
影响因子:15.1
引用产品: 786-O [786-0] 细胞, A-498 细胞, Renca [RenCa] 细胞, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×, MEM (含NEAA) 培养基, Renca [RenCa]细胞专用培养基
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Deubiquitylation and stabilization of ARMC5 by ubiquitin-specific processing protease 7 (USP7) are critical for RCC proliferation(2021)
作者:Guobei Yan, Na Liu, Jun Tian, Yanli Fu, Wei Wei, Jingjing Zou, Suping Li, Qing Wang, Kai Li, Junhua Wang
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子:5.3
引用产品: 786-O [786-0] 细胞, 769-P 细胞, A-498 细胞, ACHN 细胞, 293T [HEK-293T] 细胞
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The roles of ferroptosis regulatory gene SLC7A11 in renal cell carcinoma: A multi-omics study(2021)
作者:Fangshi Xu, Yibing Guan, Li Xue
期刊:Cancer Medicine
影响因子:4.0
引用产品: 786-O [786-0] 细胞, A-498 细胞, HK-2 细胞
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HOXD1 functions as a novel tumor suppressor in kidney renal clear cell carcinoma(2021)
作者:Yuanbo Cui, Chunyan Zhang, Ya Li
期刊:Cell Biology International
影响因子:3.9
引用产品: HK-2 细胞, A-498 细胞, 786-O [786-0] 细胞, Caki-1 细胞
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Epigenetic inactivation of hydroxymethylglutaryl CoA synthase reduces ketogenesis and facilitates tumor cell motility in clear cell renal carcinoma(2021)
作者:Peipei Han, Yifang Wang, Wenqi Luo
期刊:Pathology Research And Practice
影响因子:3.3
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APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells(2021)
作者:Hua Xia, Yamin Liu, Lei Xu
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-021-00589-z
影响因子:2.1
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Tumor promoting effects of circRNA_001287 on renal cell carcinoma through miR-144-targeted CEP55(2020)
作者:Jiafu Feng, Yongcan Guo, Yuanmeng Li
期刊:Journal Of Experimental & Clinical Cancer Research
DOI:10.1186/s13046-020-01744-2
影响因子:11.3
引用产品: A-498 细胞, Caki-1 细胞, Caki-2 细胞, 786-O [786-0] 细胞
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RETRACTED ARTICLE: Silencing circular RNA-ZNF652 represses proliferation and EMT process of renal carcinoma cells via raising miR-205(2020)
作者:Ludong Zhang, Ya Guo
期刊:Artificial Cells Nanomedicine and Biotechnology
DOI:10.1080/21691401.2020.1725532
影响因子:5.8
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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