货号: CL-0254
Price: ¥1800 ¥1800
生长培养基: MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A-498(人肾癌细胞) |
别称 | A498 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)[PM150410]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A-498细胞是由Aaronson·S建立,源自一位52岁患有肾癌的女性病人。 |
年龄(性别) | 52Y Female |
组织来源 | 肾脏 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice. |
STR位点信息
STR鉴定图
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CircSCNN1A inhibits the proliferation, migration and invasion of renal cell carcinoma cells by decreasing CLDN8 expression through miR-590-5p(2024)
作者:Tingting Guo, Wanjuan Xiong, Chong Liu, Li Zhu, Ling Xie
期刊:GENESIS
影响因子:1.5
引用产品: 786-O [786-0] 细胞, McCoy's 5A 培养基, DMEM高糖 培养基, A-498 细胞, HK-2 细胞, RPMI-1640 培养基, 769-P 细胞, Caki-1 细胞
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RNF26 up-regulates PD-L1 to regulate the cancer immune response in ccRCC(2024)
作者:WEIGANG REN, JING LI, RUIJIANG ZENG, LIANG ZHU
期刊:BIOCELL
DOI:10.32604/biocell.2024.051747
影响因子:0.8
引用产品: Renca [RenCa] 细胞, MEM(ATCC改良) 培养基, A-498 细胞, Renca [RenCa]细胞专用培养基
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ZDHHC2-mediated AGK palmitoylation activates AKT/mTOR signaling to reduce sunitinib sensitivity in renal cell carcinoma(2023)
作者:Sun Yan, Zhu Liang, Liu Pian, Zhang Huan, Guo Feng, Jin Xin
期刊:CANCER RESEARCH
DOI:10.1158/0008-5472.CAN-22-3105
影响因子:11.2
引用产品: A-498 细胞, MEM (含NEAA) 培养基, Annexin V-FITC染色液
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Serine hydroxymethyltransferase 2 knockdown induces apoptosis in ccRCC by causing lysosomal membrane permeabilization via metabolic reprogramming(2023)
作者:Liu Zhangnan, Fan Mengzhen, Hou Junqing, Pan Sijing, Xu Yanxin, Zhang Hailong, Liu Chen, Hao Xiangjun, Li Xia, Wang Huijuan
期刊:Cell Death & Disease
DOI:10.1038/s41419-023-05677-4
影响因子:9.0
引用产品: A-498 细胞, ACHN 细胞, 786-O [786-0] 细胞, Caki-1 细胞
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The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis(2023)
作者:Wang Yapeng, Peng Mou, Zhong Yawen, Xiong Wei, Zhu Liang, Jin Xin
期刊:ONCOGENE
DOI:10.1038/s41388-023-02613-w
影响因子:8.0
引用产品: 786-O [786-0] 细胞, A-498 细胞, STR鉴定, MEM (含NEAA) 培养基
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Identification of the H3K36me3 reader LEDGF/p75 in the pancancer landscape and functional exploration in clear cell renal cell carcinoma(2023)
作者:Yuwei Zhang, Wei Guo, Yangkun Feng, Longfei Yang, Hao Lin, Pengcheng Zhou, Kejie Zhao, Lin Jiang, Bing Yao, Ninghan Feng
期刊:Computational and Structural Biotechnology Journal
DOI:10.1016/j.csbj.2023.08.023
影响因子:6.0
引用产品: Caki-1 细胞, 786-O [786-0] 细胞, A-498 细胞, HK-2 细胞
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Focusing on the Abnormal Events of NPC1, NPC2, and NPC1L1 in Pan-Cancer and Further Constructing LUAD and KICH Prediction Models(2023)
作者:Keheng Chen, Xin Zhang, Guangyu Sun, Zhichao Fang, Lusheng Liao, Yanping Zhong, Fengdie Huang, Mingyou Dong, Shihua Luo
期刊:JOURNAL OF PROTEOME RESEARCH
DOI:10.1021/acs.jproteome.3c00655
影响因子:4.4
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, HK-2 细胞, A-498 细胞, MEM 培养基, RPMI-1640 培养基, Ham's F-12K 培养基
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Construction and Validation of a Novel Cuproptosis-Related Seven-lncRNA Signature to Predict the Outcomes, Immunotherapeutic Responses, and Targeted Therapy in Patients with Clear Cell Renal Cell Carcinoma(2023)
作者:Haoxun Zhang, Zikuan Ning, Bowen Wang, Yiwen Liu, Boju Tao, Guoling Zhang, Hua Liu, Chunyang Wang
期刊:DISEASE MARKERS
影响因子:3.5
引用产品: A-498 细胞, OS-RC-2 细胞
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YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway(2022)
作者:Li Wei, Ye Kun, Li Xurui, Liu Xinlin, Peng Mou, Chen Fang, Xiong Wei, Wang Yinhuai, Zhu Liang
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02460-9
影响因子:12.7
引用产品: 786-O [786-0] 细胞, A-498 细胞, STR鉴定, MEM (含NEAA) 培养基
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Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma(2022)
作者:Liangmin Fu, Jiahao Bao, Jinhui Li, Qiuyang Li, Hansen Lin, Yayun Zhou, Jiangbo Li, Yixuan Yan, Marvin E. Langston, Tianhao Sun, Songliang Guo, Xinwei Zhou, Yuhang Chen, Yujun Liu, Yiqi Zhao, Jun Lu, Yong Huang, Wei Chen, Benjamin I. Chung, Junhang Luo
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2022.1021935
影响因子:8.8
引用产品: ACHN 细胞, HK-2 细胞, Caki-2 细胞, A-498 细胞, Caki-1 细胞, 786-O [786-0] 细胞, OS-RC-2 细胞, 769-P 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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