TNF-α/ TNFA/ TNFSF2, Human, Recombinant
货号： PCK050
Price： ￥4880 ￥1780 ￥980 ￥480 ￥7080
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 别名 | Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;TNF;TNFA;TNFSF2 |
| 物种 | 人 |
| 表达宿主 | E.coli |
| 序列信息 | Val77-Leu233 |
| 检索号 | P01375 |
| 分子量 | 17.5 kDa |
| 表观分子量 | 16 kDa |
| 生物活性 | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介绍 | TNFα is a homotrimer with a subunit molecular mass of 17 kD Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease. |
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A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics(2023)
期刊：LAB ON A CHIP
影响因子：6.100
引用产品： 人脐静脉内皮细胞, TNF-α/ TNFA/ TNFSF2, Human, Recombinant, Calcein-AM/PI活死细胞双染试剂盒
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:这个重组蛋白如果我想加入细胞里，用哪个溶解呢 ？因为随产品附赠了一管pbs和一管无菌水
首次复融使用无菌水，复融后如果还需要进一步稀释的话，使用含海藻糖的PBS进行稀释。
识别码示意图
