M-CSF/CSF-1/MGI-IM, Human, Recombinant
货号： PCK042
Price： ￥14680 ￥3480 ￥1180 ￥19680
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 别名 | CSF-1;MGI-IM |
| 物种 | 人 |
| 表达宿主 | E.coli |
| 序列信息 | MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS with polyhistidine tag at the C-terminus. |
| 检索号 | P09603.2 |
| 分子量 | 13.34 kDa |
| 表观分子量 | 13/19 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human M-CSF is approximately > 2.5 × 10^8 IU/mg. |
| 纯度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介绍 | Macrophage Colony-Stimulating Factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression. |
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A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal modulation of bactericidal and anti-inflammation process in osteomyelitis treatment(2022)
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-022-01614-3
影响因子：9.429
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:这个重组蛋白如果我想加入细胞里，用哪个溶解呢 ？因为随产品附赠了一管pbs和一管无菌水
首次复融使用无菌水，复融后如果还需要进一步稀释的话，使用含海藻糖的PBS进行稀释。
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