IL-1β/IL1F2, Human, Recombinant

文献(2) 说明书
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https://789.bio/ec/qea1R0
  • https://789.bio/ec/qea1R0

货号: PCK047

Price: ¥11880 ¥2280 ¥780 ¥17280

Size:
500μg 50μg 10μg 1mg

产品信息

产品形态 粉末
产品规格 500μg / 50μg / 10μg / 1mg
别名 Catabolin;Lymphocyte-Activating Factor (LAF);Endogenous Pyrogen (EP);Leukocyte Endogenous Mediator (LEM);Mononuclear Cell Factor (MCF)
物种
表达宿主 E.coli
序列信息 MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
检索号 P01584.2
分子量 18.48 kDa
表观分子量 17 kDa
标签 His-tag at the C-terminus
生物活性 His-tag at the C-terminus
纯度 > 98% as determined by SDS-PAGE. Ni-NTA chromatography
内毒素 < 0.1 EU per 1 μg of the protein by the LAL method.
储存条件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件 Ambient temperature or ice pack.
有效期 12个月
制剂 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
背景介绍 Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.
  • Q1:官网的因子都是不含动物成分的么?

    我们公司的因子都是重组的,是不含动物源成分的。

  • Q2:这个重组蛋白如果我想加入细胞里,用哪个溶解呢 ?因为随产品附赠了一管pbs和一管无菌水

    首次复融使用无菌水,复融后如果还需要进一步稀释的话,使用含海藻糖的PBS进行稀释。

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