HT-29(人结肠癌细胞)
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货号: CL-0118
Price: ¥1600
产品信息
产品名称 | HT-29(人结肠癌细胞) |
别称 | HT 29;HT29 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:McCoy's 5A+10% FBS+1% P/S 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) | 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的;近来,已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
年龄(性别) | 44Y Female |
组织来源 | 结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
STR位点信息
STR鉴定图
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MicroRNA?9?5p increases the sensitivity of colorectal cancer cells to 5?fluorouracil by downregulating high mobility group A2 expression(2021)
期刊:Oncology Letters
影响因子:2.900
引用产品: HCT 116 细胞, HT-29 细胞, MET-5A细胞专用培养基
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Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer(2021)
期刊:Pathology Research And Practice
影响因子:2.800
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The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway(2021)
期刊:PeerJ
影响因子:2.700
引用产品: Hep G2 细胞, AGS 细胞, HCT 116 细胞, DLD-1 细胞, HT-29 细胞
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR?1269a/VASH1 axis(2021)
期刊:Experimental and Therapeutic Medicine
影响因子:2.700
引用产品: HCT 116 细胞, Caco-2 细胞, LoVo 细胞, SW480 [SW-480] 细胞, HT-29 细胞
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Indole-3-propionic Acid Improved the Intestinal Barrier by Enhancing Epithelial Barrier and Mucus Barrier(2020)
期刊:Journal Of Agricultural And Food Chemistry
影响因子:6.100
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Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition(2020)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2020.109707
影响因子:4.800
引用产品: HCT 116 细胞, LoVo 细胞, HT-29 细胞, Ham's F-12K 培养基
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miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition(2020)
期刊:Drug Design Development and Therapy
影响因子:4.319
引用产品: HCT 116 细胞, SW620 细胞, HT-29 细胞, McCoy's 5A 培养基, SW620 细胞, McCoy's 5A 培养基
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020)
期刊:Investigational New Drugs
DOI:10.1007/s10637-020-01014-0
影响因子:3.400
引用产品: HCT 116 细胞, SW480 [SW-480] 细胞, SW620 细胞, HT-29 细胞, LoVo 细胞, RKO 细胞
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Anti-cancer effects of Porphyra haitanensis polysaccharides on human colon cancer cells via cell cycle arrest and apoptosis without causing adverse effects in vitro(2020)
期刊:3 BIOTECH
DOI:10.1007/s13205-020-02379-y
影响因子:2.800
引用产品: HT-29 细胞, LoVo 细胞, SW480 [SW-480] 细胞
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SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip(2020)
期刊:Science Bulletin
DOI:10.1016/j.scib.2020.11.015
影响因子:18.900
引用产品: HT-29 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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