HT-29(人结肠癌细胞)
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货号: CL-0118
Price: ¥1600
产品信息
产品名称 | HT-29(人结肠癌细胞) |
别称 | HT 29;HT29 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:McCoy's 5A+10% FBS+1% P/S 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) | 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的;近来,已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
年龄(性别) | 44Y Female |
组织来源 | 结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
STR位点信息
STR鉴定图
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Development and therapeutic assessment of bispecific nanobodies targeting B-cell activating factor and interleukin-17 for the neutralization of inflammatory mediators in autoimmune diseases(2025)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2025.140057
影响因子:7.700
引用产品: HT-29 细胞, McCoy's 5A 培养基, Raji 细胞
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Exploring the Anti-Colorectal Cancer Mechanism of Norcantharidin Through TRAF5/NF-κB Pathway Regulation and Folate-Targeted Liposomal Delivery(2025)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子:4.900
引用产品: HT-29 细胞, HCT 116 细胞
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Inhibitory effects of κ-carrageenan oligosaccharides on histamine formation in Atlantic mackerel (Scomber scombrus) during cold storage(2025)
期刊:Food Bioscience
DOI:10.1016/j.fbio.2025.105921
影响因子:4.800
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m6A-mediated regulation of CPSF6 by METTL3 promotes oxaliplatin resistance in colorectal cancer through enhanced glycolysis(2025)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2025.111676
影响因子:4.400
引用产品: HT-29 细胞, HCT 116 细胞
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Ultrasound-Assisted Extraction of Polysaccharides from Lyophyllum decastes: Structural Analysis and Bioactivity Assessment(2025)
期刊:MOLECULES
影响因子:4.200
引用产品: HT-29 细胞, A549 [A-549] 细胞, RAW 264.7 细胞, MCF7 [MCF-7] 细胞, Hep G2 细胞
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TYMS Enhances Colorectal Cell Antioxidant Capacity Via the KEAP1-NRF2 Pathway to Resist Ferroptosis(2025)
期刊:Journal of Cancer
影响因子:3.300
引用产品: SW480 [SW-480] 细胞, HCT 116 细胞, SW620 细胞, HT-29 细胞, HCT-8 [HRT-18] 细胞, LoVo 细胞
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Investigating the role of MicroRNA-519d-3p in enhancing chemosensitivity of colorectal cancer cells to 5-Fluorouracil through PFKFB3 targeting(2025)
期刊:Clinics
DOI:10.1016/j.clinsp.2025.100606
影响因子:2.200
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NSUN2 Knockdown Promotes the Ferroptosis of Colorectal Cancer Cells Via m5C Modification of SLC7A11 mRNA(2025)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-025-11035-0
影响因子:2.100
引用产品: HT-29 细胞, HCT 116 细胞, SW480 [SW-480] 细胞, LoVo 细胞, SW620 细胞
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Mechanism underlying the role of the circRNA OMA1/miR-654-3p/RAF1 axis in children with inflammatory bowel disease(2025)
期刊:CYTOTECHNOLOGY
DOI:10.1007/s10616-025-00703-z
影响因子:2.000
引用产品: HT-29 细胞
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NGR-Modified CAF-Derived exos Targeting Tumor Vasculature to Induce Ferroptosis and Overcome Chemoresistance in Osteosarcoma(2025)
期刊:Advanced Science
影响因子:14.300
引用产品: HT-29 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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