EGF/Urogastrone/URG, Human, Recombinant
货号： PCK001
Price： ￥880 ￥680 ￥380 ￥180 ￥1080
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 别名 | Urogastrone;URG |
| 物种 | 人 |
| 表达宿主 | E.coli |
| 序列信息 | MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus. |
| 检索号 | P01133.2 |
| 分子量 | 7.16 kDa |
| 表观分子量 | 9-11 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | His-tag at the C-terminus |
| 纯度 | > 95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介绍 | Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture. |
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Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior(2023)
期刊：Small Methods
影响因子：12.400
引用产品： DMEM/F12 培养基, 马血清, EGF/Urogastrone/URG, Human, Recombinant, 重组人胰岛素溶液 (10mg/mL), MEM非必需氨基酸溶液 (NEAA)，100×, Hela 细胞, MCF 10A 细胞
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer(2022)
期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子：5.600
引用产品： PC-9 细胞, A549 [A-549] 细胞, NCI-H1299 细胞, NCI-H1975 细胞, BEAS-2B 细胞, RPMI-1640 培养基, BEAS-2B细胞专用培养基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome(2022)
期刊：EXPERIMENTAL AND MOLECULAR MEDICINE
DOI：10.1038/s12276-022-00848-3
影响因子：12.200
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Q1:EGF拿到后如何配制
先用离心机高速离心细胞因子冻干粉（离心机的最高速即可），使冻干粉全部收集到离心管底部；再用适量无菌水溶解至不低于100 μg/mL浓度，然后用无菌的完全培养基、0.1%的BSA或者5%海藻糖溶液进行进一步稀释（使用含血清、BSA或者海藻糖的溶液配制，可以有效保证蛋白活性，保存时间会更久一点）；细胞因子产品为无菌产品，不用过滤，使用无菌离心管分装后放在-20℃冰箱保存。
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Q2:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q3:这个重组蛋白如果我想加入细胞里，用哪个溶解呢 ？因为随产品附赠了一管pbs和一管无菌水
首次复融使用无菌水，复融后如果还需要进一步稀释的话，使用含海藻糖的PBS进行稀释。
识别码示意图
