MTT溶液(5 mg/mL)
货号: PB180519
Price: ¥160 ¥240
产品信息
产品简介 | MTT全称为3-(4,5-dimethyl-2-thiazolyl)-2,5-dipHenyl-2-H-tetrazolium bromide,汉语化学名为 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐,商品名:噻唑蓝,是一种黄颜色的染料。MTT能够检测细胞存活和生长,其检测原理为:活细胞线粒体中的琥珀酸脱氢酶,能使外源性MTT还原为不溶于水的蓝紫色结晶物--甲瓒(Formazan),并沉积在细胞中,而死细胞则无此功能;二甲基亚砜(DMSO)能溶解细胞中的甲瓒,用酶标仪在490nm或570nm波长处(常用490nm)测定其光吸收值(OD值),通过OD值的大小,可以判断活细胞的数量或活性,在一定细胞数范围内,MTT结晶形成的量与活细胞的数量或活性成正比。该方法的特点是灵敏度高,并且十分经济,已广泛应用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。 |
产品形态 | 液体 |
产品浓度 | 5mg/mL |
产品规格 | 1mL×5 / 1mL×10 |
PH | 7.2-7.6 |
溶剂 | 10 mM PBS |
内毒素 | <3 |
储存条件 | -5~-20℃,避光储存 |
运输条件 | 低温 |
有效期 | 18个月 |
注意事项 |
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Inhibitory Effect of Puerarin on Lipopolysaccharide-triggered Inflammatory Responses of Bovine Kidney Cells(2024)
期刊:CELL BIOCHEMISTRY AND BIOPHYSICS
DOI:10.1007/s12013-024-01303-y
影响因子:2.600
引用产品: MTT溶液(5 mg/mL)
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Effect of 5-Aza-2’-deoxycytidine on T-cell acute lymphoblastic leukemia cell biological behaviors and PTEN expression(2024)
期刊:CytoJournal
DOI:10.25259/Cytojournal_31_2024
影响因子:2.500
引用产品: DMEM高糖培养基,干粉, MTT溶液(5 mg/mL)
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Circ_0000277 promotes the malignant progression of oesophageal squamous cell carcinoma through miR-1294-dependent regulation of DVL3(2024)
期刊:Molecular & Cellular Toxicology
DOI:10.1007/s13273-024-00443-9
影响因子:1.700
引用产品: MTT溶液(5 mg/mL), 人食管上皮细胞, KYSE-150 细胞
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Rapid discovery of a novel “green” and natural GST inhibitor for sensitizing hepatocellular carcinoma to Cisplatin by visual screening strategy(2023)
期刊:Journal of Pharmaceutical Analysis
DOI:10.1016/j.jpha.2023.12.013
影响因子:8.800
引用产品: 特级胎牛血清, 0.25%胰蛋白酶溶液(含EDTA,溶于PBS), 磷酸盐缓冲液(PBS) 1×, MTT溶液(5 mg/mL), DMEM高糖 培养基
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Selection of goat β-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells(2023)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.127312
影响因子:8.200
引用产品: MTT溶液 (5mg/mL)
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DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging(2023)
期刊:JACS Au
影响因子:8.000
引用产品: DMEM/F12 培养基, 重组人胰岛素溶液 (10mg/mL), MEM非必需氨基酸溶液 (NEAA),100×, EGF/Urogastrone/URG, Human, Recombinant, 马血清, MTT溶液 (5mg/mL), MCF 10A 细胞
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Bone marrow mesenchymal stem cell exosome-derived lncRNA TUC339 influences the progression of osteoarthritis by regulating synovial macrophage polarization and chondrocyte apoptosis(2023)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.115488
影响因子:7.500
引用产品: MTT溶液 (5mg/mL)
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The role of SLC39A4 in the prognosis, immune microenvironment, and contribution to malignant behavior in vivo and in vitro of cervical cancer(2023)
期刊:Translational Oncology
DOI:10.1016/j.tranon.2023.101839
影响因子:5.000
引用产品: Hela 细胞, MEM 培养基, MTT溶液(5 mg/mL)
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Developing a Novel Enamel Adhesive with Amorphous Calcium Phosphate and Silver Nanoparticles to Prevent Demineralization during Orthodontic Treatment(2023)
期刊:Journal of Functional Biomaterials
影响因子:4.800
引用产品: MTT溶液 (5mg/mL)
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High quantum yield nitrogen-doped carbon quantum dots: Green synthesis and application as “on-off” fluorescent sensors for specific Fe3+ ions detection and cell imaging(2023)
期刊:DIAMOND AND RELATED MATERIALS
DOI:10.1016/j.diamond.2023.109702
影响因子:4.100
引用产品: MTT溶液 (5mg/mL), Hep G2 细胞, 特级胎牛血清, DMEM高糖 培养基

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