0.25%胰蛋白酶溶液(含EDTA、酚红,溶于D-Hank's)
货号: PB180226
Price: ¥268 ¥68
产品信息
产品简介 | 胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在pH 8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。 一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。 |
产品形态 | 液体 |
产品浓度 | 0.25% |
产品规格 | 500mL / 125mL |
PH | 7.2-8.0 |
EDTA·2Na | 0.2 g/L |
溶剂 | D-hank's |
酚红 | 10 mg/L |
内毒素 | <3 |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项 |
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引用产品: MC3T3-E1 Subclone 24 (新引进) 细胞, 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
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Q1:胰蛋白酶放4度放多久,我再官网上查询只有“不宜长时间放置于室温环境或2~8℃长期保存”?
如果您的用量比较少的情况,建议把胰酶分装到15mL的离心管中,留出1管放在4℃使用,剩余的放进-20℃保存,使用胰酶的时候,建议是常温复温到不冰手使用就可以。

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