货号: CL-0016
Price: ¥1400 ¥1400
生长培养基: Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A549 [A-549](人非小细胞肺癌细胞) |
别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系,源自于一位58岁白人男性;A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
年龄(性别) | 58Y Male |
组织来源 | 肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Proteasome 26S subunit, non-ATPase 1 (PSMD1) facilitated the progression of lung adenocarcinoma by the de-ubiquitination and stability of PTEN-induced kinase 1 (PINK1)(2022)
作者:Lihua Liu, Aihua Liu, Jun Dong
期刊:Experimental Cell Research
DOI:10.1016/j.yexcr.2022.113075
影响因子:3.7
引用产品: SK-MES-1 细胞, NCI-H2170 细胞, HCC827 细胞, A549 [A-549] 细胞
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CircRNA ZNF609 Knockdown Represses the Development of Non-Small Cell Lung Cancer via miR-623/FOXM1 Axis(2022)
作者:Fanghan Wang, Xiangfeng Li, Xigao Jia, Luxin Geng
期刊:Cancer Management and Research
影响因子:3.6
引用产品: HBE135-E6E7 细胞, Calu-6 细胞, A549 [A-549] 细胞, Calu-3 细胞, NCI-H1299 细胞
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Construction of a Humanized PBMC-PDX Model to Study the Efficacy of a Bacterial Marker in Lung Cancer Immunotherapy(2022)
作者:Chengwei Wu, Xinning Wang, Haitao Shang, Hong Wei
期刊:DISEASE MARKERS
影响因子:3.5
引用产品: A549 [A-549] 细胞
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Molecular mechanism of Rhubarb in the treatment of non-small cell lung cancer based on network pharmacology and molecular docking technology(2022)
作者:Tan Ye-Ru, Lu Yu
期刊:MOLECULAR DIVERSITY
DOI:10.1007/s11030-022-10501-w
影响因子:3.4
引用产品: A549 [A-549] 细胞
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IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation(2022)
作者:Wenping Liu, Miaomiao Xin, Qing Li, Linqian Sun, Xiao Han, Jibo Wang
期刊:Biomed Research International
影响因子:3.3
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, Ham's F-12K 培养基, RPMI-1640 培养基
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circRNA-mediated upregulation of HOXC9 is correlated with poor outcome and immune microenvironment infiltrates in LUAD(2022)
作者:Yunlong Liu, Liling Jing, Jie Zhang
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.10.043
影响因子:3.3
引用产品: A549 [A-549] 细胞, SK-MES-1 细胞
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METTL3 promotes non-small cell lung cancer (NSCLC) cell proliferation and colony formation in a m6A-YTHDF1 dependent way(2022)
作者:Dou Xuejun, Wang Zhiyuan, Lu Weiqiang, Miao Libin, Zhao Yuefeng
期刊:BMC Pulmonary Medicine
DOI:10.1186/s12890-022-02119-3
影响因子:3.3
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, HCC827 细胞, NCI-H1975 细胞, 人支气管上皮细胞, 青霉素-链霉素溶液 (双抗),100×
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Circ_0002476 regulates cell growth, invasion, and mtDNA damage in non–small cell lung cancer by targeting miR-1182/TFAM axis(2022)
作者:Weijie Wang, Haiting Sun, Xuan Ma, Ting Zhu, Haina Zhang
期刊:Thoracic Cancer
影响因子:3.2
引用产品: NCI-H1299 细胞, A549 [A-549] 细胞, 16HBE 细胞
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Hsa_circ_0092887 targeting miR-490-5p/UBE2T promotes paclitaxel resistance in non-small cell lung cancer(2022)
作者:Limei Wang, Zhiyong Zhang, Hui Tian
期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子:3.1
引用产品: MRC-5 细胞, DMEM高糖 培养基, 特级胎牛血清, NCI-H1299 细胞, A549 [A-549] 细胞, Ham's F-12K 培养基, RPMI-1640 培养基
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BMI1 induces ubiquitination and protein degradation of Nod-like receptor family CARD domain containing 5 and suppresses human leukocyte antigen class I expression to induce immune escape in non-small cell lung cancer(2022)
作者:Zhi-Hui Lu, Gan-Jie Tu, Si-Lv Fu, Kai Shang, Su-Juan Peng, Li Chen, Xi-Juan Gu
期刊:KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子:3.0
引用产品: 293T [HEK-293T] 细胞, 16HBE 细胞, A549 [A-549] 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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