货号： CL-0732
Price： ￥1800 ￥1800
生长培养基： 90% EpiCM[CM-ZY021]+4% EpiCGS[CM-ZY022]+5% FBS[CM-ZY013]+1% P/S[CM-ZY014]
产品信息
| 产品名称 | HBE135-E6E7（人支气管上皮细胞） |
| 别称 | HBE 135-E6E7; HBE 135-E6/E7 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样；卵圆形 |
| 冻存条件 |
冻存液：55% DM/F12+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:90% EpiCM[CM-ZY021]+4% EpiCGS[CM-ZY022]+5% FBS[CM-ZY013]+1% P/S[CM-ZY014] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/mL G418. |
| 年龄（性别） | 54Y Male |
| 组织来源 | 肺；支气管 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
STR位点信息
STR鉴定图
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Phospholipids of inhaled liposomes determine the in vivo fate and therapeutic effects of salvianolic acid B on idiopathic pulmonary fibrosis(2024)
作者：Jianqing Peng, Qin Wang, Runbin Sun, Ke Zhang, Yi Chen, Zipeng Gong
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2024.05.026
影响因子：10.8
引用产品： A549 [A-549] 细胞, HBE135-E6E7 细胞, 人支气管上皮细胞完全培养基, RAW 264.7 细胞
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A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma(2024)
作者：Qinglin Wang, Xuming Song, Yijian Zhang, Si Liang, Minhao Zhang, Hui Wang, Yipeng Feng, Rutao Li, Hanlin Ding, Yuzhong Chen, Wenjie Xia, Gaochao Dong, Lin Xu, Qixing Mao, Feng Jiang
期刊：Cell Reports
DOI：10.1016/j.celrep.2024.114550
影响因子：7.5
引用产品： HBE135-E6E7 细胞
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Fine particulate matter promotes airway inflammation and mucin production by activating endoplasmic reticulum stress and the IRE1α/NOD1/NF‑κB pathway(2023)
作者：Lihua Hu, Chaoqun Xu, Xiang Tang, Shanjun Yu, Lijun Wang, Qi Li, Xiangdong Zhou
期刊：INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子：5.4
引用产品： HBE135-E6E7 细胞
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Comprehensive analysis of BUBs gene family in lung adenocarcinoma with immunological analysis(2023)
作者：Xiaojuan Li, Tianqi Wang, Mi Li, Xing Bao, Tian Ma, Caihong Yang, Hua Wu, Hao Li
期刊：Aging-US
影响因子：5.2
引用产品： HBE135-E6E7 细胞
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Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1(2023)
作者：Shaofeng Xia, Chenliang Wang
期刊：International Journal of Genomics
影响因子：2.9
引用产品： 293T [HEK-293T] 细胞, NCI-H460 [H460] 细胞, A549 [A-549] 细胞, HBE135-E6E7 细胞
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Hsa_circ_0003489 Drives PTX Resistance of Human NSCLC Cells Through Modulating miR-98-5p/IGF2(2023)
作者：Shaofeng Xia, Chenliang Wang
期刊：Pharmacogenomics & Personalized Medicine
影响因子：1.9
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Low-density lipoprotein receptor-related protein 8 facilitates the proliferation and invasion of non-small cell lung cancer cells by regulating the Wnt/β-catenin signaling pathway(2022)
作者：Zhi Fang, Min Zhong, Ling Zhou
期刊：Bioengineered
DOI：10.1080/21655979.2022.2036917
影响因子：4.9
引用产品： HBE135-E6E7 细胞
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer(2022)
作者：Feiyun Chang , Jiali Li , Quan Sun
期刊：Bioengineered
DOI：10.1080/21655979.2022.2053810
影响因子：4.9
引用产品： 293T [HEK-293T] 细胞, NCI-H460 [H460] 细胞, NCI-H1650 细胞, A549 [A-549] 细胞, NCI-H1299 细胞, HBE135-E6E7 细胞
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CircRNA ZNF609 Knockdown Represses the Development of Non-Small Cell Lung Cancer via miR-623/FOXM1 Axis(2022)
作者：Fanghan Wang, Xiangfeng Li, Xigao Jia, Luxin Geng
期刊：Cancer Management and Research
影响因子：3.6
引用产品： HBE135-E6E7 细胞, Calu-6 细胞, A549 [A-549] 细胞, Calu-3 细胞, NCI-H1299 细胞
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Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis(2022)
作者：Huafen Zi, Li Chen, Qian Ruan
期刊：Histology And Histopathology
影响因子：2.0
引用产品： HBE135-E6E7 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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