货号: CL-0016
Price: ¥1400 ¥1400
生长培养基: Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A549 [A-549](人非小细胞肺癌细胞) |
别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系,源自于一位58岁白人男性;A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
年龄(性别) | 58Y Male |
组织来源 | 肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Synthesis and antitumor activities of 4-(1H-indol-1-yl)-4-oxobutanoic acid spliced podophyllotoxin derivatives(2023)
作者:Jun Yang, Guang-Ping Liang, Xiong-Li Liu
期刊:SYNTHETIC COMMUNICATIONS
DOI:10.1080/00397911.2023.2164863
影响因子:2.1
引用产品: A549 [A-549] 细胞, A549/DDP 细胞, MCF7 [MCF-7] 细胞, HL-7702 [L-02; LO2] 细胞
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circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway(2023)
作者:Changpeng Sun, Hongjun Guan, Jinjin Li, Yinfeng Gu
期刊:Open Medicine
影响因子:2.1
引用产品: 16HBE 细胞, NCI-H522[H522] 细胞, A549 [A-549] 细胞, RPMI-1640 培养基, Ham's F-12K 培养基, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×
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Ginsenoside Rg3 enhances the radiosensitivity of lung cancer A549 and H1299 cells via the PI3K/AKT signaling pathway(2023)
作者:Li Jialin, Yang Bo
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-023-00749-3
影响因子:2.1
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, RPMI-1640 培养基, STR鉴定
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Propofol-mediated circ_0000735 downregulation restrains tumor growth by decreasing integrin-β1 expression in non-small cell lung cancer(2023)
作者:Lihui Zhang, Yunli Gao, Yue Li, Xinying Li, Haixia Gong
期刊:Open Medicine
影响因子:2.1
引用产品: 人支气管上皮细胞, NCI-H1299 细胞, A549 [A-549] 细胞, 293T [HEK-293T] 细胞, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×
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Circ_0079530 stimulates THBS2 to promote the malignant progression of non-small cell lung cancer by sponging miR-584-5p(2023)
作者:Kun Fang, Yibin Deng, Ping Yang, Yurong Zhang, Dan Luo, Fang Wang, Zhilong Cai, Yang Liu
期刊:HISTOLOGY AND HISTOPATHOLOGY
影响因子:2.0
引用产品: 16HBE 细胞, NCI-H1299 细胞, NCI-H460 [H460] 细胞, A549 [A-549] 细胞, Ham's F-12K 培养基, 293T [HEK-293T] 细胞
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Hsa_circ_0003489 Drives PTX Resistance of Human NSCLC Cells Through Modulating miR-98-5p/IGF2(2023)
作者:Shaofeng Xia, Chenliang Wang
期刊:Pharmacogenomics & Personalized Medicine
影响因子:1.9
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A Systematic Study of Traditional Chinese Medicine for the Treatment of Lung Adenocarcinoma Using a Reverse Network of Key Targets Based on Bioinformatics and Molecular Docking: Curcumin and Trans-Resveratrol as Potential Drug Candidates for Lung Adenocarcinoma(2023)
作者:Wujun Wang, Zhu Zeng, Junli Zhang, Zengtao Sun
期刊:Natural Product Communications
影响因子:1.8
引用产品: A549 [A-549] 细胞, Ham's F-12K 培养基
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Resveratrol suppresses NSCLC cell growth, invasion and migration by mediating Wnt/β-catenin pathway via downregulating SIX4 and SPHK2(2023)
作者:Xiaolan Wang, Caixia Liu, Jian Wang, Zexiang Tian
期刊:JOURNAL OF CHEMOTHERAPY
DOI:10.1080/1120009X.2023.2281759
影响因子:1.8
引用产品: NCI-H1299 细胞, A549 [A-549] 细胞, HBE 细胞
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Circ_0030411 aggravates cisplatin-resistance in non-small cell lung cancer by serving as a miR-495-3p sponge to enhance CCND1 expression(2023)
作者:Jing Zhang, Xiaohai Cui, Hangying Qu, Yunfeng Zhang
期刊:JOURNAL OF CHEMOTHERAPY
DOI:10.1080/1120009X.2022.2162218
影响因子:1.8
引用产品: 16HBE 细胞, A549 [A-549] 细胞, NCI-H460 [H460] 细胞, 293T [HEK-293T] 细胞
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METTL14 knockdown inhibits the pyroptosis in the sepsis-induced acute lung injury through regulating the m6A modification of NLRP3(2023)
作者:Jianting Gao, Huizhen Chen, Yong Zhang, Sun Yu, Yiyi Wu, Qiuyan Wang, Zhenfei Yu
期刊:EXPERIMENTAL LUNG RESEARCH
DOI:10.1080/01902148.2023.2288182
影响因子:1.7
引用产品: A549 [A-549] 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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