货号： CL-0833
Price： ￥2500 ￥2500
生长培养基： 90% EpiCM[CM-ZY021]+4% EpiCGS[CM-ZY022]+5% FBS[CM-ZY013]+1% P/S[CM-ZY014]
产品信息
| 产品名称 | THLE-2（人肝永生化细胞） |
| 别称 | THLE2; Transformed Human Liver Epithelial-2 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% DM/F12+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:90% EpiCM[CM-ZY021]+4% EpiCGS[CM-ZY022]+5% FBS[CM-ZY013]+1% P/S[CM-ZY014] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways. |
| 年龄（性别） | Adult Male |
| 组织来源 | Liver; Left lobe |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 致瘤性 | No; No, nude mice. |
STR位点信息
STR鉴定图
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CircTTC13 promotes sorafenib resistance in hepatocellular carcinoma through the inhibition of ferroptosis by targeting the miR-513a-5p/SLC7A11 axis(2025)
作者：Zhang Ying, Yao Ruiwei, Li Mingyi, Fang Chongkai, Feng Kunliang, Chen Xiuru, Wang Jinan, Luo Rui, Shi Hanqian, Chen Xinqiu, Zhao Xilin, Huang Hanlin, Liu Shuwei, Yin Bing, Zhong Chong
期刊：Molecular Cancer
DOI：10.1186/s12943-024-02224-3
影响因子：27.7
引用产品： THLE-2 细胞
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Disturbing Cholesterol/Sphingolipid Metabolism by Squalene Epoxidase Arises Crizotinib Hepatotoxicity(2025)
作者：Hao Yan, Xiangliang Huang, Yourong Zhou, Yuan Mu, Shaoyin Zhang, Yashi Cao, Wentong Wu, Zhifei Xu, Xueqin Chen, Xiaochen Zhang, Xiaohong Wang, Xiaochun Yang, Bo Yang, Qiaojun He, Peihua Luo
期刊：Advanced Science
影响因子：14.3
引用产品： THLE-2 细胞, THLE-2细胞专用培养基
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Revealing Molecular Connections between Dissolved Organic Matter in Surface Water Sources and Their Cytotoxicity Influenced by Chlorination Disinfection(2025)
作者：Huihui Hong, Hai Huang, Sheng-Ao Li, Jinxian Lin, Kun Luo, Xinghong Cao, Fuyi Cui, Zhou Zhou, Hua Ma
期刊：ENVIRONMENTAL SCIENCE & TECHNOLOGY
影响因子：10.8
引用产品： THLE-2 细胞
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The promoting effect of the POU3F2/METTL16/PFKM cascade on glycolysis and tumorigenesis of hepatocellular carcinoma: Role of the POU3F2/METTL16/PFKM axis in HCC(2025)
作者：Ming Chen, Yuan Yang, Guangsheng Hu, Zhong Peng, Wu Wen
期刊：Annals of Hepatology
DOI：10.1016/j.aohep.2025.101776
影响因子：3.7
引用产品： THLE-2 细胞, THLE-2细胞专用培养基
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Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway(2025)
作者：Yushuang Xing, Di Jia, Xinping Zhu, Jialu Yang, Zhipeng Gao, Nana Meng, Haohao Xu, Mengxiao Wang, Shijun Chang, Mingqian Zhao, Shanbo Zhang, Zichen Mu, Qiang Tang, Weiming Zhao
期刊：PLoS One
DOI：10.1371/journal.pone.0318450
影响因子：2.9
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Accumulation of TOX high mobility group box family member 3 promotes the oncogenesis and development of hepatocellular carcinoma through the MAPK signaling pathway(2024)
作者：Yufu Peng, Jing Yu, Fei Liu, Leyi Tang, Bo Li, Wei Zhang, Kefei Chen, Haili Zhang, Yonggang Wei, Xuelei Ma, Hubing Shi
期刊：MedComm
DOI：10.1002/mco2.510
影响因子：9.9
引用产品： THLE-2 细胞
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O-GlcNAcylated RALY Contributes to Hepatocellular Carcinoma Cells Proliferation by Regulating USP22 mRNA Nuclear Export(2024)
作者：Liu Shiwei, Lv Qingpeng, Mao Xinyu, Dong Hui, Xu Wenjing, Du Xuanlong, Jia Weilu, Feng Kun, Zhang Jiaqi, Zhang Yewei
期刊：International Journal of Biological Sciences
影响因子：8.2
引用产品： HuH-7 细胞, THLE-2 细胞, Hep 3B2.1-7 细胞
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RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling(2024)
作者：Fang Yuan, Tang WeiQiang, Qu Siming, Li ZhiTao, Zhang XiaoLi, Miao YingLei, Zeng Zhong, Huang HanFei
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-024-04964-8
影响因子：7.4
引用产品： Hep 3B2.1-7 细胞, HuH-7 细胞, PLC/PRF/5 细胞, Li-7 细胞, THLE-2 细胞
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Development of cantharidin/baicalin co-delivery system based on mitochondrial targeting strategy for enhanced hepatocellular carcinoma therapy(2024)
作者：Li Huang, Zhonglan Yang, Yuan He, Lei Yang, Wangzhong Xiao, Jialuo Cai, Hongqiao Fan, Yilin Xu, Xinhua Xia
期刊：Applied Materials Today
DOI：10.1016/j.apmt.2024.102374
影响因子：7.2
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TEAD1, MYO7A and NDUFC2 are novel functional genes associated with glucose metabolism in BXD recombinant inbred population(2024)
作者：Yingying Wu, Chao Zhang, Shaofei Duan, Yushan Li, Lu Lu, Akhilesh Bajpai, Chunhua Yang, Jia Mi, Geng Tian, Fuyi Xu, Donglai Qi, Zhaowei Xu, Xiao Dong Chi
期刊：DIABETES OBESITY & METABOLISM
影响因子：5.8
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Q1:THLE-2 细胞，客户问用药后，细胞要经过饥饿处理，这个过程中细胞不能用完全培养基，应该用什么培养。
可以使用DMEM-F12培养基
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Q2:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
识别码示意图
