货号： CL-0710
Price： ￥2500 ￥2500
生长培养基： DMEM[PM150210]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | MC3T3-E1 Subclone 24（小鼠胚胎成骨细胞前体细胞） |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:DMEM[PM150210]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3天/次 |
参考资料(来源文献)
| 背景描述 | MC3T3-E1 Subclone 24细胞有多个亚克隆，可以作为体外研究成骨细胞分化的良好模型，尤其是ECM信号通路的作用。 |
| 年龄（性别） | <1D Sex unspecified |
| 组织来源 | 颅骨 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in immunosuppressed mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Construction of Magnesium Phosphate Chemical Conversion Coatings with Different Microstructures on Titanium to Enhance Osteogenesis and Angiogenesis(2024)
作者：Yi-bo Li, Huan-qing Zhang, Yu-peng Lu, Xiao-juan Yang, Guan-duo Wang, Yu-ying Wang, Kang-le Tang, Sheng-yun Huang, Gui-yong Xiao
期刊：ACS Applied Materials & Interfaces
影响因子：8.3
引用产品： MC3T3-E1 Subclone 14 细胞, 特级胎牛血清
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ZIF-8-Modified Black Phosphorus Nanosheets Incorporated into Injectable Dual-Component Hydrogels for Enhanced Photothermal Antibacterial and Osteogenic Activities(2024)
作者：Quan Li, Ruijie Wang, Jinfang Xue, Ruiyu Wang, Shun Zhang, Hai Kang, Yang Wang, Huadong Zhu, Chuanzhu Lv
期刊：ACS Applied Materials & Interfaces
影响因子：8.3
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Tumor-oriented and chemo-photothermal nanoplatform capable of sensitizing chemotherapy and ferroptosis against osteosarcoma metastasis(2024)
作者：Hongzi Wu, Bowen Li, Jichun Shao, Qingquan Kong
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.132019
影响因子：8.2
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Surface functionalization of calcium magnesium phosphate cements with alginate sodium for enhanced bone regeneration via TRPM7/PI3K/Akt signaling pathway(2024)
作者：Changtian Gong, Jian Yang, Xiping Zhang, Xingyu Wang, Zicheng Wei, Xinghan Huang, Weichun Guo
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.130998
影响因子：8.2
引用产品： MC3T3-E1 Subclone 14 细胞
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The Dual Angiogenesis Effects via Nrf2/HO-1 Signaling Pathway of Melatonin Nanocomposite Scaffold on Promoting Diabetic Bone Defect Repair(2024)
作者：Tingting Chen, Zimei Wu, Qiaodan Hou, Yixin Mei, Kunkun Yang, Jing Xu, Lin Wang
期刊：International Journal of Nanomedicine
影响因子：8.0
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An injectable carboxymethyl chitosan-based hydrogel with controlled release of BMP-2 for efficient treatment of bone defects(2024)
作者：Tianyi Zhou, Fei Wang, Kunyu Liu, Haiyan Zhou, Jian Shang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.137120
影响因子：7.7
引用产品： MEMα 培养基, MC3T3-E1 Subclone 14 细胞
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Microporous polylactic acid/chitin nanocrystals composite scaffolds using in-situ foaming 3D printing for bone tissue engineering(2024)
作者：Kangming Peng, Shihao Chen, Velmurugan Senthooran, Xueling Hu, Yi Qi, Chen Zhang, Lixin Wu, Jianlei Wang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.135055
影响因子：7.7
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A Mechanistic Study of the Osteogenic Effect of Arecoline in an Osteoporosis Model: Inhibition of Iron Overload-Induced Osteogenesis by Promoting Heme Oxygenase-1 Expression(2024)
作者：Zhongjing Jiang, Linhua Deng, Gang Xiang, Xia Xu, Yunjia Wang
期刊：Antioxidants
影响因子：7.0
引用产品： MC3T3-E1 Subclone 14 细胞
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Yam Carbon Dots Promote Bone Defect Repair by Modulating Histone Demethylase 4B(2024)
作者：QianYang Chen, Shuo Liu, Yuhan Wang, MeiChen Tong, HaiBo Sun, Ming Dong, Yun Lu, WeiDong Niu, LiNa Wang
期刊：International Journal of Nanomedicine
影响因子：6.6
引用产品： MC3T3-E1 Subclone 14 细胞
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Study on the Antibacterial Activity and Bone Inductivity of Nanosilver/PLGA-Coated TI-CU Implants(2024)
作者：Zhaoli Geng, Renping Dong, Xinlin Li, Xinyi Xu, Lin Chen, Xu Han, Dongxu Liu, Yi Liu
期刊：International Journal of Nanomedicine
影响因子：6.6
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
识别码示意图
