货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Regulation of Bone Remodeling by Metal–Phenolic Networks for the Treatment of Systemic Osteoporosis(2025)
作者:Xi Chen, Weihui Wu, Wei Zhu, Jiajing Zhou, Jingqu Chen, Zhixing Lin, Sixian Zhang, Frank Caruso, Changsheng Liu
期刊:ACS Applied Materials & Interfaces
影响因子:8.3
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Mass transport characteristic and biocompatibility of demand-guided structural biomaterials(2025)
作者:Yang Zhao, Qianqian Wu, Linzhi Wu
期刊:COMPOSITE STRUCTURES
DOI:10.1016/j.compstruct.2025.118848
影响因子:6.3
引用产品: MC3T3-E1 Subclone 14 细胞
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5,7-Dihydroxy-4-Methylcoumarin enhances osteogenesis and ameliorates osteoporosis via the AKT1 pathway(2025)
作者:Han Diao, Houzhi Yang, Bin Yu, Yonggang Fan, Shenao Li, Jigeng Fan, Donglun Xiao, Ying Zhao, Miao Guo, Ying Zhang, Shaoyuan Huang, Qiong Tang, Shan-shan Li, Tianwei Sun, Xin Jin
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子:5.3
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3D-Printed PCL/SrHA@DFO Bone Tissue Engineering Scaffold with Bone Regeneration and Vascularization Function(2025)
作者:Kai Chen, Liu Luo, Ruolan Tao, Muzi Li, Shuqi Qu, Xiaofang Wu, Xinyue Zhang, Haiyan Feng, Ziqiang Zhu, Dekun Zhang
期刊:ACS Applied Bio Materials
影响因子:4.6
引用产品: MC3T3-E1 Subclone 14 细胞
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Activation of osteoblast ferroptosis by risperidone accelerates bone loss in mice models of schizophrenia(2025)
作者:Fan Hongyan, Yang Zaihong, Pang Lan, Li Peifan, Duan Changrong, Xia Guangyuan, Zheng Lei
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-025-05520-w
影响因子:2.8
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Dual disease co-expression analysis reveals potential roles of estrogen-related genes in postmenopausal osteoporosis and Parkinson’s disease(2025)
作者:Dongdong Yu, Jian Kang, Chengguo Ju, Qingyan Wang, Ye Qiao, Long Qiao, Dongxiang Yang
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2024.1518471
影响因子:2.8
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Effects of ADRM1 on osteoblast differentiation and mineralization in osteoporosis(2025)
作者:Huafeng Zhuang, Yongjun Lin, Chengye Lin, Miao Zheng, Xuedong Yao, Youjia Xu
期刊:American Journal of Translational Research
影响因子:1.7
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Upconversion 3d Bioprinting for Noninvasive In-Vivo Moulding(2024)
作者:Peng Zhang, Zhaowei Teng, Min Zhou, Xue Yu, Hongyu Wen, Junzheng Niu, Zhichao Liu, Zhimeng Zhang, Yang Liu, Jianbei Qiu, Xuhui Xu
期刊:ADVANCED MATERIALS
影响因子:29.4
引用产品: MC3T3-E1 Subclone 14 细胞
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A clinical-stage Nrf2 activator suppresses osteoclast differentiation via the iron-ornithine axis(2024)
作者:Yimin Dong, Honglei Kang, Renpeng Peng, Zheming Liu, Fuben Liao, Shi-an Hu, Weizhong Ding, Pengju Wang, Pengchao Yang, Meipeng Zhu, Sibo Wang, Minglong Wu, Dawei Ye, Xin Gan, Feng Li, Kehan Song
期刊:Cell Metabolism
DOI:10.1016/j.cmet.2024.03.005
影响因子:29.0
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Gene-Activating Framework Nucleic Acid-Targeted Upregulating Sirtuin-1 to Modulate Osteoimmune Microenvironment for Diabetic Osteoporosis Therapeutics(2024)
作者:Zhengwen Cai, Long Bai, Qiumei Li, Yong Li, Xiaoxiao Cai, Yunfeng Lin
期刊:ACS Nano
影响因子:15.8
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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