货号： CL-0710
Price： ￥2500 ￥2500
生长培养基： DMEM[PM150210]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | MC3T3-E1 Subclone 24（小鼠胚胎成骨细胞前体细胞） |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:DMEM[PM150210]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3天/次 |
参考资料(来源文献)
| 背景描述 | MC3T3-E1 Subclone 24细胞有多个亚克隆，可以作为体外研究成骨细胞分化的良好模型，尤其是ECM信号通路的作用。 |
| 年龄（性别） | <1D Sex unspecified |
| 组织来源 | 颅骨 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in immunosuppressed mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Apocynin exerts cytoprotective effects on dexamethasone-induced osteoblasts by inhibiting oxidative stress through the Nrf2 signalling pathway(2023)
作者：Xinglong Zhang, Ran Pang, Kai Zhang, Qian Xu, Chunlei Xu, Wei Shi, Xinyu Liang, Dong Li, Wenhao Cui, Shucai Bai, Zhijun Li, Hui Li, Huafeng Zhang
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子：5.3
引用产品： MC3T3-E1 Subclone 14 细胞
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Fish skin gelatin-based adhesive hydrogels loading cod peptides with osteogenic activity for bone tissue engineering(2023)
作者：Wuchao Ma, Meilian Yang, Di Wu, Yao Li, Li-Shu Wang, Hesham R. El-Seedi, Chao Wu, Ming Du
期刊：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
DOI：10.1016/j.colsurfa.2023.131695
影响因子：5.2
引用产品： MC3T3-E1 Subclone 14 细胞
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Physicochemical and biological properties of composite bone cement based on octacalcium phosphate and tricalcium silicate(2023)
作者：Jiaxi Liu, Fan Qiu, Yuhan Zou, Zilong Zhang, Anping Wang, Yin Zhang
期刊：CERAMICS INTERNATIONAL
DOI：10.1016/j.ceramint.2023.03.155
影响因子：5.2
引用产品： MC3T3-E1 Subclone 24 细胞
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Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro, and Anti-Bacterial Properties(2023)
作者：Jiayu He, Yuandong Liu, Xiaofeng Zeng, Yan Tong, Run Liu, Kan Wang, Xiangdong Shangguan, Guanzhou Qiu, Coswald Stephen Sipaut
期刊：Journal of Functional Biomaterials
影响因子：4.8
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Osteogenic activity of a micro/nano hierarchical nano-hydroxyapatite coating on zirconium alloy(2023)
作者：Xiaoning Xu, Xiaohui Sun, Yueyan Tian, Ligang Zhang, Libin Liu
期刊：MATERIALS CHARACTERIZATION
DOI：10.1016/j.matchar.2023.113356
影响因子：4.7
引用产品： MC3T3-E1 Subclone 14 细胞
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Direct Knockdown of PDZ and LIM Domain 1 Using an Adenoviral Delivery System Accelerates Osteogenesis and Fracture Healing in Mice(2023)
作者：Dr. Zhiguang Chen, Ms. Qi Zhao, Mr. Ran Zhang, Dr. Lianghong Chen, Mr. Songlan Gao, Dr. Yu Wang, Dr. Tiegang Li, Dr. Jinqi Xue
期刊：HUMAN GENE THERAPY
影响因子：4.2
引用产品： MC3T3-E1 Subclone 24 细胞
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Preparation, characterization and antioxidant activity of cobalt polysaccharides from Qingzhuan Dark Tea.(2023)
作者：Hongfu Zhou, Yong Chen, Ziyao Wang, Chen Xie, Dan Ye, Anran Guo, Wenjing Xie, Jun Xing, Min Zheng
期刊：Heliyon
DOI：10.1016/j.heliyon.2023.e15503
影响因子：4.0
引用产品： MC3T3-E1 Subclone 24 细胞
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Bone marrow fatty acids affect osteoblastic differentiation through miR-92b-3p in the early stages of postmenopausal osteoporosis(2023)
作者：Sizhu Wang, Cuisong Tang, Jieying Chen, Huan Tang, Lin Zhang, Guangyu Tang
期刊：Heliyon
DOI：10.1016/j.heliyon.2023.e16513
影响因子：4.0
引用产品： MC3T3-E1 Subclone 14 细胞
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Integrating network pharmacology and experimental verification strategies to reveal the active ingredients and molecular mechanism of Tenghuang Jiangu Capsule against osteoporosis(2023)
作者：Miao Li, Hongyu Tang, Yuanhao Hu, Songtao Li, Pan Kang, Baihao Chen, Shaocong Li, Meng Zhang, Haibin Wang, Shaochuan Huo
期刊：Heliyon
DOI：10.1016/j.heliyon.2023.e19812
影响因子：4.0
引用产品： MC3T3-E1 Subclone 14 细胞
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Macrophage-derived apoptotic bodies impair the osteogenic ability of osteoblasts in periodontitis(2023)
作者：Xu Liu, Lijia Guo, Juan Du, Zhenhua Luo, Junji Xu, Ujjal Kumar Bhawal, Xiaoyan Li, Yi Liu
期刊：ORAL DISEASES
影响因子：3.8
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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