货号： CL-0843
Price： ￥2000 ￥2000
生长培养基： 90% EpiCM[GCM-ZY011]+4% EpiCGS[GCM-ZY012]+5% FBS[GCM-ZY013]+1% P/S[GCM-ZY014]
产品信息
| 产品名称 | hCMEC/D3（人脑微血管内皮细胞） |
| 别称 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 纺锤形细胞 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:90% EpiCM[GCM-ZY011]+4% EpiCGS[GCM-ZY012]+5% FBS[GCM-ZY013]+1% P/S[GCM-ZY014] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 年龄（性别） | Adult Female |
| 组织来源 | 脑微血管 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
STR位点信息
STR鉴定图
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A critical role for microglia in regulating metabolic homeostasis and neural repair after spinal cord injury(2024)
作者：Huan Jian, Kailin Wu, Yigang Lv, Jiawei Du, Mengfan Hou, Chi Zhang, Jianqing Gao, Hengxing Zhou, Shiqing Feng
期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2024.10.288
影响因子：7.1
引用产品： SH-SY5Y [SHSY-5Y] 细胞, hCMEC/D3 细胞
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A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell(2024)
作者：Xiaohui Wang, Xinran Li, Baoquan Wang, Yanxia Xie, Xueru Cui, Jin Jiao, Jianwei Jiao, Yachao Zhang
期刊：ANALYTICA CHIMICA ACTA
影响因子：5.7
引用产品： U251 细胞, NIH/3T3 细胞, SCC7 细胞, 4T1 细胞, B16-F10 细胞, Hela 细胞, Hep G2 细胞, LN229 [LN-229; LNT-229] 细胞, PC-9 细胞, LLC [LL/2; LLC1] 细胞, hCMEC/D3 细胞, HL-7702 [L-02; LO2] 细胞
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Reactive astrocytes promote tumor progression by up-regulating tumor protocadherin 1 expression in lung cancer brain metastasis(2024)
作者：Mengyi Tang, Kun Liang, Wenzhe Duan, Shengkai Xia, Dongmei Shi, Encheng Li, Wenwen Liu, Qi Wang
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2024.150431
影响因子：2.5
引用产品： hCMEC/D3 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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