货号: CL-0402
Price: ¥1800 ¥1800
生长培养基: RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | NCI-H520(人肺鳞癌细胞) |
别称 | H520; H-520; NCI-HUT-520; NCIH520 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | NCI-H520细胞是由Gazdar AF等在1982年建系而来,源于鳞状细胞肺癌组织;NCI-H520细胞p53 mRNA表达水平较正常肺组织低,但是没有大的结构DNA的异常。NCI-H520细胞角蛋白、波形蛋白阳性,神经丝三联蛋白阴性;NCI-H520细胞在软琼脂中可形成克隆。 |
年龄(性别) | Age unspecified Male |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10<sup>7</sup> cells (Tumors developed within 21 days at 100% frequency (5/5)). |
STR位点信息
STR鉴定图
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HVEM as a Tumor-Intrinsic Regulator in Non-Small Cell Lung Cancer: Suppression of Metastasis via Glycolysis Inhibition and Modulation of Macrophage Polarization(2025)
作者:Yuanshan Yao, Bin Li, Chunji Chen, Jing Wang, Feng Yao, Zhigang Li
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2025.107604
影响因子:9.1
引用产品: A549 [A-549] 细胞, NCI-H1975 细胞, NCI-H1299 细胞, NCI-H226 [H226] 细胞, PC-9 细胞, NCI-H520 细胞, NCI-H1563 细胞, BEAS-2B 细胞, THP-1 细胞
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Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer(2024)
作者:Han Fushi, Chen Shuzhen, Zhang Kangwei, Zhang Kunming, Wang Meng, Wang Peijun
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-024-05057-2
影响因子:7.4
引用产品: NCI-H2228 [H2228; H-2228] 细胞, NCI-H520 细胞, 16HBE 细胞
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TFAP2C-mediated transcriptional activation of STEAP3 promotes lung squamous cell carcinoma progression by regulating the β-catenin pathway(2024)
作者:Sun Tong, Yang Zhiguang
期刊:Biology Direct
DOI:10.1186/s13062-024-00584-w
影响因子:5.7
引用产品: NCI-H1703 细胞, SK-MES-1 细胞, NCI-H520 细胞, NCI-H226 [H226] 细胞
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Development of a disulfidptosis-related lncRNA prognostic signature for enhanced prognostic assessment and therapeutic strategies in lung squamous cell carcinoma(2024)
作者:Zhu Ankang, Zong Yan, Gao Xingcai
期刊:Scientific Reports
DOI:10.1038/s41598-024-68423-6
影响因子:3.8
引用产品: NCI-H520 细胞, BEAS-2B 细胞
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Liquiritigenin Induces Cell Cycle Arrest and Apoptosis in Lung Squamous Cell Carcinoma(2024)
作者:Liu Yaqi, Wang Yixiao, Yang Yiran, Quan Yihong, Guo Mingxing
期刊:CELL BIOCHEMISTRY AND BIOPHYSICS
DOI:10.1007/s12013-024-01294-w
影响因子:2.6
引用产品: NCI-H520 细胞, SK-MES-1 细胞
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Increased LOXL2 expression is related to poor prognosis in lung squamous cell carcinoma(2024)
作者:Lei Cao, Jian Zhong, Zicheng Liu, Jie Jiang, Chenyao Zhu, Feng Liu, Bo Wang
期刊:Journal of Thoracic Disease
影响因子:2.1
引用产品: NCI-H226 [H226] 细胞, NCI-H520 细胞
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Effects of 630?nm laser on apoptosis, metastasis, invasion and epithelial-to-mesenchymal transition of human lung squamous cell carcinoma H520 cells mediated by hematoporphyrin derivatives(2024)
作者:Liu Tingting, Zhang Enhua, Cui Shichao, Dai Haoyu, Yang Xiaohui, Lin Cunzhi
期刊:LASERS IN MEDICAL SCIENCE
DOI:10.1007/s10103-024-04176-y
影响因子:2.1
引用产品: NCI-H520 细胞, 青霉素-链霉素溶液(双抗),100×, 特级胎牛血清, RPMI-1640 培养基, 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
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Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer(2023)
作者:Keying Liang, Qian Wang, Li Qiu, Xiaocheng Gong, Zixi Chen, Haibo Zhang, Ke Ding, Yunfei Liu, Jinfen Wei, Shudai Lin, Shuying Fu, Hongli Du
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子:5.6
引用产品: HCC1937 细胞, NCI-H520 细胞, SNU-387 细胞, SNU-182 细胞, BEAS-2B 细胞, MCF 10A细胞专用培养基
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Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation(2023)
作者:Xiaochen Ni, Shilong Yu, Xiaomin Jiang, Feng Wu, Jun Zhou, Defang Mao, Haibo Wang, Yujian Tao, Yanqing Liu, Feng Jin
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子:5.4
引用产品: NCI-H226 [H226] 细胞, NCI-H520 细胞, HCC827 细胞
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Development and validation of a N6 methylation regulator-related gene signature for prognostic and immune response prediction in non-small cell lung cancer(2023)
作者:Xiang Li, Jinlong Ma, Zhenqian Sun, Na Li, Guangjun Jiao, Tianqi Zhang, Hongxin Cao
期刊:American Journal of Cancer Research
DOI:PMID:37560004
影响因子:5.3
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, NCI-H520 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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