货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Novel antiosteoporotic peptides purified from protein hydrolysates of taihe black-boned silky fowl: By larval zebrafish model and molecular docking(2023)
作者:Yang Qiu, Jianyue Ying, Fujie Yan, Huilin Yu, Yan Zhao, Honghao Li, Shengyao Xia, Jianchu Chen, Jiajin Zhu
期刊:FOOD RESEARCH INTERNATIONAL
DOI:10.1016/j.foodres.2023.112850
影响因子:8.1
引用产品: MC3T3-E1 Subclone 14 细胞
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Isosinensetin alleviates estrogen deficiency-induced osteoporosis via suppressing ROS-mediated NF-κB/MAPK signaling pathways(2023)
作者:Yiwu Qin, Dezhi Song, Shijie Liao, Junchun Chen, Minglian Xu, Yuangang Su, Haoyu Lian, Hui Peng, Linhua Wei, Kai Chen, Jiake Xu, Jinmin Zhao, Qian Liu
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.114347
影响因子:7.5
引用产品: MC3T3-E1 Subclone 14 细胞
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Corylifol A protects against ovariectomized-induced bone loss and attenuates RANKL‐induced osteoclastogenesis via ROS reduction, ERK inhibition, and NFATc1 activation(2023)
作者:Yinglong Xu, Dezhi Song, Xixi Lin, Hui Peng, Yuangang Su, Jiamin Liang, Na Hai, Jinmin Zhao, Qian Liu
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2023.01.017
影响因子:7.4
引用产品: MC3T3-E1 Subclone 14 细胞
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Application of steam explosion treatment on the collagen peptides extraction from cattle bone(2023)
作者:Hongru Zhang, Hong Liu, Liwei Qi, Xiong Xv, Xia Li, Yujie Guo, Wei Jia, Chunhui Zhang, Aurore Richel
期刊:Innovative Food Science & Emerging Technologies
DOI:10.1016/j.ifset.2023.103336
影响因子:6.6
引用产品: MC3T3-E1 Subclone 24 细胞, MEMα 培养基, 特级胎牛血清
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Wettability transition of superhydrophobic myristic acid/dodecyl mercaptan/Cu@ZIF-8/HA coating modified magnesium alloy driven by visible light for antibacterial and osteogenic applications(2023)
作者:Lei Ling, Shu Cai, You Zuo, Tengfei Meng, Hao Tian, Xiaogang Bao, Guohua Xu
期刊:PROGRESS IN ORGANIC COATINGS
DOI:10.1016/j.porgcoat.2023.107828
影响因子:6.6
引用产品: MC3T3-E1 Subclone 24 细胞
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Myosin heavy chain-derived peptide of Gadus morhua promotes proliferation and differentiation in osteoblasts and bone formation and maintains bone homeostasis in ovariectomized mice(2023)
作者:Meilian Yang, Shuzhen Cheng, Wuchao Ma, Di Wu, Hesham R. El-Seedi, Zhenyu Wang, Ming Du
期刊:Food & Function
影响因子:6.1
引用产品: MC3T3-E1 Subclone 14 细胞
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Poly(β-amino ester) Dual-Drug-Loaded Hydrogels with Antibacterial and Osteogenic Properties for Bone Repair(2023)
作者:Lu Lv, Wanting Cheng, Sining Wang, Sihui Lin, Jiarui Dang, Zhihui Ran, Hong Zhu, Wenjin Xu, Zhijun Huang, Peihu Xu, Haixing Xu
期刊:ACS Biomaterials Science & Engineering
DOI:10.1021/acsbiomaterials.2c01524
影响因子:5.8
引用产品: MC3T3-E1 Subclone 24 细胞
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Stability, biomechanics and biocompatibility analysis following different preparation strategies of hierarchical zeolite coatings on titanium alloy surfaces(2023)
作者:Chang Liu, Jiaxin Zhang, Xin Zhao, Mingwei Xu, He Liu, Hongming Zhou
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2023.1337709
影响因子:5.7
引用产品: MC3T3-E1 Subclone 14 细胞
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Ti3C2Tx MXene-incorporated macroporous bacterial cellulose scaffolds with physical and chemical crosslinking for enhanced mechanical and biological performance(2023)
作者:Yang Zhiwei, Zhang Yichuan, Chen Yuqin, Yang Zhengzhao, Cui Teng, Luo Honglin, Liu Yue, Wan Yizao
期刊:CELLULOSE
DOI:10.1007/s10570-023-05254-0
影响因子:5.7
引用产品: MC3T3-E1 Subclone 14 细胞
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Dipeptidyl peptidase 3 is essential for maintaining osteoblastic differentiation under a high-glucose environment by inhibiting apoptosis, oxidative stress and inflammation through the modulation of the Keap1-Nrf2 pathway(2023)
作者:Xiaofeng Li, Xiangyou Yu, Shenglin He, Jing Li
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110404
影响因子:5.6
引用产品: MC3T3-E1 Subclone 14 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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