货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
-
Filamin B knockdown impairs differentiation and function in mouse pre-osteoblasts via aberrant transcription and alternative splicing(2024)
作者:Xi Wang, Qiyu Jia, Li Yu, Jinyong Huang, Xin Wang, Lijun Zhou, Wubulikasimu Mijiti, Zhenzi Xie, Shiming Dong, Zengru Xie, Hairong Ma
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e39334
影响因子:3.4
引用产品: MC3T3-E1 Subclone 14 细胞
-
Biomolecule-grafted GO enhanced the mechanical and biological properties of 3D printed PLA scaffolds with TPMS porous structure(2024)
作者:Xiaotong Ye, Enyu Wang, Yanjian Huang, Yanjuan Yang, Tianwen Zhang, Hui You, Yu Long, Wang Guo, Bin Liu, Shan Wang
期刊:Journal of the Mechanical Behavior of Biomedical Materials
DOI:10.1016/j.jmbbm.2024.106646
影响因子:3.3
引用产品: MC3T3-E1 Subclone 14 细胞
-
Optimisation of recovery protein hydrolysate from chicken bone by steam explosion strategy and its calcium-binding capacity and potential pro-osteogenic activity(2024)
作者:Xiong Xu, Liwei Qi, Hongjun Li, Chunhui Zhang
期刊:INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
影响因子:3.3
引用产品: MC3T3-E1 Subclone 14 细胞
-
Supplementation with carnosine, a food-derived bioactive dipeptide, alleviates dexamethasone-induced oxidative stress and bone impairment via the NRF2 signaling pathway(2024)
作者:Xi-you Li, Xiao-yuan Gu, Xiao-min Li, Jian-gang Yan, Xin-liang Mao, Qin Yu, Yu-lan Du, Hiroshi Kurihara, Chang-Yu Yan, Wei-xi Li
期刊:JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
影响因子:3.3
引用产品: MC3T3-E1 Subclone 14 细胞
-
Unraveling IGFBP3-mediated m6A modification in fracture healing(2024)
作者:Aining Lai, Junjian Sun, Zhiyuan Dai, Long Guo, Degang Tao, Haitang Li, Bin Chen, Rong Zhou
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子:2.8
引用产品: MC3T3-E1 Subclone 14 细胞
-
p-Smad3 differentially regulates the cytological behavior of osteoclasts before and after osteoblasts maturation(2024)
作者:Ye Jiapeng, Hua Zhen, Xiao Jirimutu, Shao Yang, Li Shaoshuo, Yin Heng, Wu Mao, Rong Yi, Hong Bowen, Guo Yang, Ma Yong, Wang Jianwei
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-024-09400-0
影响因子:2.8
引用产品: MC3T3-E1 Subclone 14 细胞, 小鼠骨髓单核细胞
-
FNDC5/irisin ameliorates bone loss of type 1 diabetes by suppressing endoplasmic reticulum stress?mediated ferroptosis(2024)
作者:Dong Qianqian, Han Ziqi, Gao Mingdong, Tian Limin
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-024-04701-3
影响因子:2.6
引用产品: MC3T3-E1 Subclone 14 细胞
-
Qiang Jin Mixture Promotes Osteogenic Differentiation of MC3T3-E1 Cells via BMP2/Smads Pathway and its Network Pharmacology Study(2024)
作者:Gong Weiyue, Zhu Yao, Wang Limin
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01313-4
影响因子:2.4
引用产品: MEMα 培养基, MC3T3-E1 Subclone 14 细胞
-
Natural micropatterned fish scales combing direct osteogenesis and osteoimmunomodulatory functions for enhancing bone regeneration(2023)
作者:Di Qin, Xinguo You, Haonan Wang, Yixuan Liu, Yan Shi, Na Wang, Xin Zhang, Chao Feng, Ya Liu, Ming Kong, Xiaojie Cheng, Shichao Bi, Xiguang Chen
期刊:COMPOSITES PART B-ENGINEERING
DOI:10.1016/j.compositesb.2023.110620
影响因子:13.1
-
An oxidized dextran-composite self-healing coated magnesium scaffold reduces apoptosis to induce bone regeneration(2023)
作者:Zewen Shi, Fang Yang, Yiwei Hu, Qian Pang, Lin Shi, Tianyu Du, Yuhao Cao, Baiyang Song, Xueqiang Yu, Zhaoxun Cao, Zhewei Ye, Chen Liu, Rongyao Yu, Xianjun Chen, Yabin Zhu, Qingjiang Pang
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121666
影响因子:11.2
引用产品: MC3T3-E1 Subclone 14 细胞, 人脐静脉内皮细胞
-
Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

识别码示意图