货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Osteo-angiogenic activity of a micro/nano hierarchical SrSi-codoped hydroxyapatite coating on zirconium alloy(2024)
作者:Xiaoning Xu, Kaige Wang, Xinrong Tan, Ligang Zhang, Libin Liu
期刊:Journal of Materials Research and Technology-JMR&T
DOI:10.1016/j.jmrt.2024.05.067
影响因子:6.4
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Effect of Acetyl tributyl citrate on bone metabolism based on network toxicology and molecular docking technology(2024)
作者:Xuan Lin, Kun Lin, Yue Lai, Qingping Peng, Miao Xu, Yiting Xu, Jialin Yang, Huan Liu, Jianlin Shen
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.117434
影响因子:6.2
引用产品: MC3T3-E1 Subclone 14 细胞
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High-performance bioceramic coatings of 3D printed titanium alloys via FS-auxiliary micro-arc oxidation manufacturing(2024)
作者:Yazhou Hu, Hongshui Wang, Donghui Wang, Baoe Li, Chunyong Liang
期刊:Journal of Manufacturing Processes
DOI:10.1016/j.jmapro.2024.03.095
影响因子:6.2
引用产品: MC3T3-E1 Subclone 14 细胞
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Corrosion resistance and biocompatibility of magnesium alloy with bioactive glass-reinforced hydrogel composite coatings(2024)
作者:Rong Xu, Wuxiang Zhang, Zhenzong Shao, Yong Wang, Baolin Xu, Chenkai Zhu, Xiaochuan Wu, Chi Zhang, Qianli Gao, Bin Yu, Baiwen Hu, Dongdong Xia
期刊:Journal of Materials Research and Technology-JMR&T
DOI:10.1016/j.jmrt.2024.10.111
影响因子:6.2
引用产品: MC3T3-E1 Subclone 14 细胞
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3D-Printed Scaffolds of Porous Amorphous Calcium Phosphate Nanospheres Loaded with Quercetin for Promoting Bone Repair via Synergistic Osteogenesis and Immunoregulation(2024)
作者:Rui Xu, Jie Yin, Lan Li, Ruchen Hong, Yan Chen, Qing Zhao, Yuan Zhou, Ting-ting Huang, Jin-xin Lin
期刊:ACS Applied Nano Materials
影响因子:5.9
引用产品: MC3T3-E1 Subclone 14 细胞
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Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling(2024)
作者:Chao Shan, Zeyu Wu, Yuning Xia, Xiaowei Ji, Wenjie Zhang, Xian Peng, Jin Zhao
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.112299
影响因子:5.6
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Interleukin-4-Loaded Heparin Hydrogel Regulates Macrophage Polarization to Promote Osteogenic Differentiation(2024)
作者:Yuhao Zhao, Xiaofei Feng, Zhenrui Zhao, Zhengdong Song, Wenji Wang, Haiyan Zhao
期刊:ACS Biomaterials Science & Engineering
DOI:10.1021/acsbiomaterials.4c00589
影响因子:5.4
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3,3′-Diindolylmethane promotes bone formation – A assessment in MC3T3-E1 cells and zebrafish(2024)
作者:Ying Ma, Yin Zhu, Feng Wang, Guoyang Zhao, Lianlian Huang, Rongzhu Lu, Dongxu Wang, Xinyu Tian, Yang Ye
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子:5.3
引用产品: MC3T3-E1 Subclone 14 细胞
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Mechanical properties and biological activity of 3D printed silicon nitride materials(2024)
作者:Xiaofeng Zeng, Coswald Stephen Sipaut, Noor Maizura Ismail, Yuandong Liu, Yan yan Farm, Jiayu He
期刊:CERAMICS INTERNATIONAL
DOI:10.1016/j.ceramint.2024.02.041
影响因子:5.2
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CDK14 is regulated by IGF2BP2 and involved in osteogenic differentiation via Wnt/β-catenin signaling pathway in vitro(2024)
作者:Zimo Zhou, Zhuoru Jin, Yicheng Tian, Chenghao Huangfu, Zheng Fan, Da Liu
期刊:LIFE SCIENCES
影响因子:5.2
引用产品: MC3T3-E1 Subclone 14 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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