货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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Construction of Magnesium Phosphate Chemical Conversion Coatings with Different Microstructures on Titanium to Enhance Osteogenesis and Angiogenesis(2024)
作者:Yi-bo Li, Huan-qing Zhang, Yu-peng Lu, Xiao-juan Yang, Guan-duo Wang, Yu-ying Wang, Kang-le Tang, Sheng-yun Huang, Gui-yong Xiao
期刊:ACS Applied Materials & Interfaces
影响因子:8.3
引用产品: MC3T3-E1 Subclone 14 细胞, 特级胎牛血清
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Surface functionalization of calcium magnesium phosphate cements with alginate sodium for enhanced bone regeneration via TRPM7/PI3K/Akt signaling pathway(2024)
作者:Changtian Gong, Jian Yang, Xiping Zhang, Xingyu Wang, Zicheng Wei, Xinghan Huang, Weichun Guo
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.130998
影响因子:8.2
引用产品: MC3T3-E1 Subclone 14 细胞
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High Strength and Shape Memory Spinal Fusion Device for Minimally Invasive Interbody Fusions(2024)
作者:Min Liu, Bo Liu, Ziyang Liu, Zhen Yang, Thomas J Webster, Huan Zhou, Lei Yang
期刊:International Journal of Nanomedicine
影响因子:8.0
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Exerkine FNDC5/irisin-enriched exosomes promote proliferation and inhibit ferroptosis of osteoblasts through interaction with Caveolin-1(2024)
作者:Lin Tao, Jinpeng Wang, Ke Wang, Qichang Liu, Hongyang Li, Site Xu, Chunjian Gu, Yue Zhu
期刊:AGING CELL
影响因子:8.0
引用产品: C2C12 细胞, MC3T3-E1 Subclone 14 细胞
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The Dual Angiogenesis Effects via Nrf2/HO-1 Signaling Pathway of Melatonin Nanocomposite Scaffold on Promoting Diabetic Bone Defect Repair(2024)
作者:Tingting Chen, Zimei Wu, Qiaodan Hou, Yixin Mei, Kunkun Yang, Jing Xu, Lin Wang
期刊:International Journal of Nanomedicine
影响因子:8.0
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An injectable carboxymethyl chitosan-based hydrogel with controlled release of BMP-2 for efficient treatment of bone defects(2024)
作者:Tianyi Zhou, Fei Wang, Kunyu Liu, Haiyan Zhou, Jian Shang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.137120
影响因子:7.7
引用产品: MEMα 培养基, MC3T3-E1 Subclone 14 细胞
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Effects of the combination of Epimedii Folium and Ligustri Lucidi Fructus on apoptosis and autophagy in SOP rats and osteoblasts via PI3K/AKT/mTOR pathway(2024)
作者:Yuman Li, Ping Yu, Yingying Gao, Zitong Ma, Han Wang, Yuting Long, Zaina Ma, Renhui Liu
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.116346
影响因子:7.5
引用产品: MC3T3-E1 Subclone 14 细胞
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A Mechanistic Study of the Osteogenic Effect of Arecoline in an Osteoporosis Model: Inhibition of Iron Overload-Induced Osteogenesis by Promoting Heme Oxygenase-1 Expression(2024)
作者:Zhongjing Jiang, Linhua Deng, Gang Xiang, Xia Xu, Yunjia Wang
期刊:Antioxidants
影响因子:7.0
引用产品: MC3T3-E1 Subclone 14 细胞
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Yam Carbon Dots Promote Bone Defect Repair by Modulating Histone Demethylase 4B(2024)
作者:QianYang Chen, Shuo Liu, Yuhan Wang, MeiChen Tong, HaiBo Sun, Ming Dong, Yun Lu, WeiDong Niu, LiNa Wang
期刊:International Journal of Nanomedicine
影响因子:6.6
引用产品: MC3T3-E1 Subclone 14 细胞
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Chitosan/Sodium Tripolyphosphate/Sodium Alginate/miRNA-34c-5p Antagomir Scaffolds Promote the Functionality of Rabbit Cranial Parietal Repair(2024)
作者:Chen Lin, Xinyi Bai, Linkun Zhang
期刊:International Journal of Nanomedicine
影响因子:6.6
引用产品: MC3T3-E1 Subclone 14 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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