货号: CL-0378
Price: ¥1600 ¥1600
生长培养基: MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14) |
别称 | MC3T3-E1 SUBCLONE 14 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEMα[PM150421]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4 mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | <1D Sex unspecified |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
STR位点信息
STR鉴定图
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ROS-reactive PMS/PC drug delivery system improves new bone formation under diabetic conditions by promoting angiogenesis-osteogenesis coupling via down-regulating NOX2-ROS signalling axis(2022)
作者:Zimei Wu, Qiaodan Hou, Tingting Chen, Xingzhu Jiang, Lulu Wang, Jing Xu, Lin Wang
期刊:BIOMATERIALS
DOI:10.1016/j.biomaterials.2022.121900
影响因子:15.3
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Irregular pore size of degradable bioceramic Voronoi scaffolds prepared by stereolithography: Osteogenesis and computational fluid dynamics analysis(2022)
作者:Jialiang Li, Dong Guo, Jie Li, Xinyu Wei, Zhongwei Sun, Baohui Yang, Teng Lu, Pengrong Ouyang, Su'e Chang, Weiwei Liu, Xijing He
期刊:MATERIALS & DESIGN
DOI:10.1016/j.matdes.2022.111414
影响因子:9.4
引用产品: MC3T3-E1 Subclone 24 细胞
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The osteogenesis and the biologic mechanism of thermo-responsive injectable hydrogel containing carboxymethyl chitosan/sodium alginate nanoparticles towards promoting osteal wound healing(2022)
作者:Zewen Shi, Fang Yang, Qian Pang, Yiwei Hu, Haijian Wu, Xueqiang Yu, Xianjun Chen, Lin Shi, Bowen Wen, Rui Xu, Ruixia Hou, Dan Liu, Qingjiang Pang, Yabin Zhu
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.10.142
影响因子:8.0
引用产品: 人脐静脉内皮细胞, MC3T3-E1 Subclone 14 细胞
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IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA(2022)
作者:Zimo Zhou, Senxiang Chen, Tong Wu, Yifeng Chen, Yuxiao Cao, Ying Huang, Da Liu
期刊:JOURNAL OF CELLULAR PHYSIOLOGY
影响因子:6.5
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Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells(2022)
作者:Hongru Zhang, Liwei Qi, Xiaodan Wang, Yujie Guo, Jiqian Liu, Yang Xu, Chengjiang Liu, Chunhui Zhang, Aurore Richel
期刊:Food & Function
影响因子:6.3
引用产品: MC3T3-E1 Subclone 24 细胞, MEMα 培养基, 特级胎牛血清, 小鼠骨髓间充质干细胞成骨诱导分化培养基
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Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration(2022)
作者:Huang Jianhao, Lu Jingwei, Liu Ziying, Jin Jing, Xie Chunmei, Zheng Yang, Wang Zhen, Yu Lingfeng, Zhu Yan, Fan Gentao, Sun Guojing, Xu Zhihong, Zhou Guangxin
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2022.1003677
影响因子:6.1
引用产品: MC3T3-E1 Subclone 14 细胞
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Association between SEMA3A signaling pathway genes and BMD/OP risk: An epidemiological and experimental study.(2022)
作者:Hao-long Zhou, Mu-hong Wei, Dong-sheng Di, Ru-yi Zhang, Jian-li Zhang, Ting-ting Yuan, Qian Liu, Ting-ting Zhou, Qin Huang, Qi Wang
期刊:Frontiers in Endocrinology
DOI:10.3389/fendo.2022.1014431
影响因子:6.1
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Canagliflozin promotes osteoblastic MC3T3-E1 differentiation via AMPK/RUNX2 and improves bone microarchitecture in type 2 diabetic mice(2022)
作者:Song Peiyang, Chen Tianyi, Rui Shunli, Duan Xiaodong, Deng Bo, Armstrong David G., Ma Yu, Deng Wuquan
期刊:Frontiers in Endocrinology
DOI:10.3389/fendo.2022.1081039
影响因子:6.1
引用产品: D-葡萄糖溶液(200 g/L), MC3T3-E1 Subclone 14 成骨诱导分化培养基, MC3T3-E1 Subclone 14 细胞
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Strong adhesive and drug-loaded hydrogels for enhancing bone–implant interface fixation and anti-infection properties(2022)
作者:Xingyue Wang, Xu Fang, Xin Gao, Hao Wang, Shihuai Li, Chen Li, Yunan Qing, Yanguo Qin
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2022.112817
影响因子:6.0
引用产品: MC3T3-E1 Subclone 24 细胞
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Bi-functionalization of titanium with a mixture of peptides for improving its osteogenic and antibacterial activity(2022)
作者:Qiang Tang, Weina Wang, Xiaojing Zhang, Xingtang Fang, Chunlei Zhang, Zhaojing Zhu, Yanhua Hou, Min Lai
期刊:Colloid and Interface Science Communications
DOI:10.1016/j.colcom.2022.100673
影响因子:5.6
引用产品: MC3T3-E1 Subclone 24 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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