货号： CL-0602
Price： ￥1600 ￥1600
生长培养基： DMEM/F12[PM150312]+10% FBS[164210]+0.5% ITS-G（100×）[PB180429]+40 ng/mL Dexamethasone+1% P/S[PB180120]
产品信息
| 产品名称 | AML12（小鼠正常肝细胞） |
| 别称 | AML-12; AML 12; Alpha Mouse Liver 12 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:DMEM/F12[PM150312]+10% FBS[164210]+0.5% ITS-G（100×）[PB180429]+40 ng/mL Dexamethasone+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. |
| 年龄（性别） | 3M Male |
| 组织来源 | 肝脏 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | NO |
STR位点信息
STR鉴定图
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Targeted delivery of polymeric NO-donor micelles to hepatic stellate cells for restoration of liver function and inhibition of hepatic fibrosis(2025)
作者：Rui Yang, Lifen Yang, Ni Zhang, Yuqing Wan, Shineng Chen, Yiqing Xiao, Xiaoping Liang, Shangjie Yang, Yinan Zhong, Dechun Huang, Wei Chen, Bingbing Zhao
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2025.01.036
影响因子：10.5
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1-phenyl-3-methyl-5-pyrazolone activates the AMPK pathway to alleviate western-diet induced metabolic dysfunction-associated steatohepatitis in mice(2025)
作者：Xiaoning Chen, Jiaofeng Huang, Yanying You, Hanxin Xue, Lisha Wu, Danyi Zeng, Qingqing Xing, Minxia Wu, Mingfang Wang, Jinshui Pan, Su Lin, Yueyong Zhu
期刊：CLINICAL NUTRITION
DOI：10.1016/j.clnu.2024.12.033
影响因子：6.6
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Betaine-homocysteine methyltransferase attenuates liver ischemia–reperfusion injury by targeting TAK1(2025)
作者：Xudong Liu, Jihua Shi, Min Wu, Jie Gao, Yi Zhang, Wenzhi Guo, Shuijun Zhang
期刊：FASEB JOURNAL
影响因子：4.4
引用产品： AML12 细胞, 293T [HEK-293T] 细胞, AML12细胞专用培养基
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Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway(2025)
作者：Yushuang Xing, Di Jia, Xinping Zhu, Jialu Yang, Zhipeng Gao, Nana Meng, Haohao Xu, Mengxiao Wang, Shijun Chang, Mingqian Zhao, Shanbo Zhang, Zichen Mu, Qiang Tang, Weiming Zhao
期刊：PLoS One
DOI：10.1371/journal.pone.0318450
影响因子：2.9
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Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation(2024)
作者：Zhigang Nian, Yingchao Dou, Yiqing Shen, Jintang Liu, Xianghui Du, Yong Jiang, Yonggang Zhou, Binqing Fu, Rui Sun, Xiaohu Zheng, Zhigang Tian, Haiming Wei
期刊：IMMUNITY
DOI：10.1016/j.immuni.2024.08.015
影响因子：25.5
引用产品： AML12 细胞, RAW 264.7 细胞
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Self-Sulfhydrated, Nitro-Fixed Albumin Nanoparticles as a Potent Therapeutic Agent for the Treatment of Acute Liver Injury(2024)
作者：Yanan Wang, Xiaohu Liu, Keyang Li, Xinyuan Wang, Xue Zhang, Deyao Qian, Xinlei Meng, Liangmin Yu, Xuefeng Yan, Zhiyu He
期刊：ACS Nano
影响因子：15.8
引用产品： AML12 细胞
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Lung-Targeted Lipid Nanoparticle-Delivered siUSP33 Attenuates SARS-CoV-2 Replication and Virulence by Promoting Envelope Degradation(2024)
作者：Yuzheng Zhou, Yujie Liao, Lujie Fan, Xiafei Wei, Qiang Huang, Chuwei Yang, Wei Feng, Yezi Wu, Xiang Gao, Xiaotong Shen, Jian Zhou, Zanxian Xia, Zheng Zhang
期刊：Advanced Science
影响因子：14.3
引用产品： LLC [LL/2; LLC1] 细胞, AML12 细胞
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Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury(2024)
作者：Qiwen Yu, Jiakai Zhang, Jiye Li, Yaodong Song, Jie Pan, Chaopeng Mei, Mengwei Cui, Qianqian He, Haifeng Wang, Huihui Li, Bo Cheng, Yan Zhang, Wenzhi Guo, Changju Zhu, Sanyang Chen
期刊：Advanced Science
影响因子：14.3
引用产品： AML12 细胞
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Hepatic Steatosis Aggravates Vascular Calcification via Extracellular Vesicle-Mediated Osteochondrogenic Switch of Vascular Smooth Muscle Cells(2024)
作者：Zhao-Lin Zeng, Zhi-Bo Zhao, Qing Yuan, Shi-Qi Yang, Zhen-Xing Wang, Zuo Wang, Shi-Yu Zeng, An-Qi Li, Qian Chen, Guo-Qiang Zhu, Xin-Hua Xiao, Guang-Hua Luo, Hai-Yan Luo, Jiao-Yang Li, Xu-Yu Zu, Hui Xie, Jiang-Hua Liu
期刊：Advanced Science
影响因子：14.3
引用产品： AML12 细胞
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SNRK modulates mTOR-autophagy pathway for liver lipid homeostasis in MAFLD(2024)
作者：Shan Lin, Xiusheng Qiu, Xiaoying Fu, Shuting Zhang, Changyong Tang, Jian Kuang, Haixia Guan, Shuiqing Lai
期刊：MOLECULAR THERAPY
DOI：10.1016/j.ymthe.2024.11.016
影响因子：12.1
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Q1:AML 12(小鼠正常肝细胞)DMEM/F12＋10%FBS＋0.5%ITS-G＋40ng/mlDexamethasone＋1%P/S这个加ITS-G0.5%就可以呀，我看您这个产品是100x的，那500ml体系里面是加2.5ml就行对吗？
是的，500mL培养基里加ITS-G（PB180429）2.5ml就可以。
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Q2:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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