货号： CL-0118
Price： ￥1600 ￥1600
生长培养基： McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | HT-29（人结肠癌细胞） |
| 别称 | HT 29; HT29 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的；近来，已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
| 年龄（性别） | 44Y Female |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
| 受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
STR位点信息
STR鉴定图
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Formyl peptide receptor 1 mitigates colon inflammation and maintains mucosal homeostasis through the inhibition of CREB-C/EBPβ-S100a8 signaling(2024)
作者：Tingting Li, Xiaojun Zhou, Qian Zhang, Qi Miao, Owen L Woodman, Yuguo Chen, Chengxue Qin
期刊：Mucosal Immunology
DOI：10.1016/j.mucimm.2024.04.001
影响因子：8.0
引用产品： McCoy's 5A 培养基, Caco-2 细胞, HT-29 细胞
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Hsa_circ_0007718 facilitates the progression of colorectal cancer by regulating the miR-1299/PSMC2 axis(2024)
作者：Yi Wang, Yanxia Liu, Yong Wang, Peng Ren, Hui Tian, Lin Wang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.136537
影响因子：7.7
引用产品： HT-29 细胞, SW620 细胞, NCM460 细胞, HCT 116 细胞, SW480 [SW-480] 细胞
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VWA2 protein molecular mechanism predicts colorectal cancer: Promoting cell invasion and migration by inhibiting NK cell activation(2024)
作者：Yang Tian, Qi Zhao, Haowa Wu, Jiaqi Guo, Huaxing Wu
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.135394
影响因子：7.7
引用产品： DLD-1 细胞, HCT 116 细胞, HT-29 细胞, LoVo 细胞
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Colon cancer inhibitory properties of Caulerpa lentillifera polysaccharide and its molecular mechanisms based on three-dimensional cell culture model(2024)
作者：Xuewei Xia, Yulin Wu, Zexin Chen, Danyi Du, Xiaodan Chen, Rongxin Zhang, Jun Yan, Io Nam Wong, Riming Huang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.131574
影响因子：7.7
引用产品： HT-29 细胞, HCT 116 细胞, LoVo 细胞
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Discovery of Thiazole Carboxamides as Novel Vanin-1 Inhibitors for Inflammatory Bowel Disease Treatment(2024)
作者：Tao Xie, Gao-Yao Cao, Shize Zhang, Meng-Ke Li, Xin Jin, Liu Liu, Guangji Wang, Le Zhen
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.4c01838
影响因子：6.8
引用产品： McCoy's 5A 培养基, HT-29 细胞
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DDR1 is identified as an immunotherapy target for microsatellite stable colon cancer by CRISPR screening(2024)
作者：Wu Miaoqing, Ma Wenjuan, Lv Guangzhao, Wang Xin, Li Cong, Chen Xiang, Peng Xiaofei, Tang Chaoming, Pan Zhizhong, Liu Ranyi, Chen Gong, Zhang Rongxin
期刊：npj Precision Oncology
DOI：10.1038/s41698-024-00743-2
影响因子：6.8
引用产品： HT-29 细胞, CT26.WT 细胞, DLD-1 细胞, HCT 116 细胞
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Discovery of Covalent MLKL PROTAC Degraders via Optimization of a Theophylline Derivative Ligand for Treating Necroptosis(2024)
作者：Shang Li, Liangliang Ma, Xinxin Li, Yuhan Jiang, Zhongwen Luo, Fucheng Yin, Yonglei Zhang, Yifan Chen, Siyuan Wan, Han Zhou, Lingyi Kong, Xiaobing Wang
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.4c00949
影响因子：6.8
引用产品： HT-29 细胞, U-937 细胞, J774A.1 细胞, NCTC clone 929 [L cell, L-929] 细胞
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ABL1‒YAP1 axis in intestinal stem cell activated by deoxycholic acid contributes to hepatic steatosis(2024)
作者：Tiancheng Mao, Xianjun Xu, Leheng Liu, Yulun Wu, Xiaowan Wu, Wenlu Niu, Dandan You, Xiaobo Cai, Lungen Lu, Hui Zhou
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-024-05865-6
影响因子：6.1
引用产品： HT-29 细胞
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Enhanced pharmacological activities of AKR1C3-activated prodrug AST-3424 in cancer cells with defective DNA repair(2024)
作者：Fanying Meng, Tianyang Qi, Xing Liu, Yizhi Wang, Jibing Yu, Zhaoqiang Lu, Xiaohong Cai, Anrong Li, Don Jung, Jianxin Duan
期刊：INTERNATIONAL JOURNAL OF CANCER
影响因子：5.7
引用产品： NCI-H460 [H460] 细胞, HT-29 细胞
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Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis(2024)
作者：Zihe Chen, Jueming Chen, Dongguo Lin, Hui Kang, Yanzhang Luo, Xiaogang Wang, Lihui Wang, Dayu Liu
期刊：ACS Biomaterials Science & Engineering
DOI：10.1021/acsbiomaterials.4c00727
影响因子：5.4
引用产品： SW480 [SW-480] 细胞, HCT 116 细胞, HT-29 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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