OE19(人食管癌细胞)

https://789.bio/ec/quP8dD
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货号: CL-0754

Price: ¥1800 ¥1800

Size:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

生长培养基: RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]

产品信息

产品名称 OE19(人食管癌细胞)
别称 OE-19; JROECL 19; JROECL19; OEC19
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]
培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃
培养方案B(可选) 生长培养基:
培养条件: 气相:, 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3天/次

参考资料(来源文献)

背景描述 OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2. All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
年龄(性别) 72Y Male
组织来源 食管癌
细胞类型 肿瘤细胞
肿瘤类型 食管癌细胞
生物安全等级 BSL-1
致瘤性 裸小鼠致癌

STR位点信息

STR鉴定图

  • Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?

    部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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