货号： CL-0752
Price： ￥1500 ￥1500
生长培养基： Waymouth's MB 752/1[PM151215]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | DMS 114（人肺癌细胞） |
| 别称 | DMS-114; DMS114; Darmouth Medical School 114 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:Waymouth's MB 752/1[PM151215]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
| 年龄（性别） | 68Y Male |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
STR位点信息
STR鉴定图
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NDRG2 acts as a negative regulator of the progression of small-cell lung cancer through the modulation of the PTEN-AKT-mTOR signalling cascade(2024)
作者：Zhenchuan Ma, Yuefeng Ma, Jie Feng, Zhengshui Xu, Chuantao Cheng, Jie Qin, Shaomin Li, Jiantao Jiang, Ranran Kong
期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.116915
影响因子：3.8
引用产品： NCI-H446 [H446] 细胞, NCI-H146 [H146] 细胞, NCI-H209 [H209] 细胞, DMS 114 细胞, H1688 细胞
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Zinc finger protein 367 exerts a cancer-promoting role in small cell lung cancer by influencing the CIT/LATS2/YAP signaling cascade(2024)
作者：Ranran Kong, Yuefeng Ma, Wendeng Li, Zhengshui Xu, Songyu Gong, Aoran Liu, Chuantao Cheng, Xinwu Zhang, Jie Qin, Shaomin Li, Jie Feng, Jiantao Jiang
期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.117005
影响因子：3.3
引用产品： NCI-H146 [H146] 细胞, NCI-H446 [H446] 细胞, NCI-H209 [H209] 细胞, NCI-H1688 细胞, DMS 114 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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