NCTC clone 929 [L cell, L-929](小鼠成纤维细胞)
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货号: CL-0137
Price: ¥1600
套餐组合价
产品信息
产品名称 | NCTC clone 929 [L cell, L-929](小鼠成纤维细胞) |
别称 | NCTC 929;NCTC-929;NCTC929;NCTC-929L;L cell;L cells;L-cell;L-cells;L cell line;L;Strain L-929;L-929;L 929;L929;L929(NCTC);Clone 929;Earles's cells;Earle's L cells |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)+10% FBS+1% P/S 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) | 培养条件: 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | NCTC clone 929 [L cell, L-929, derivative of Strain L-929]细胞是1948年3月建立的细胞系L的克隆株;细胞系L是最早建立的连续培养细胞系之一,而NCTC clone 929 [L cell, L-929, derivative of Strain L-929]是最早的克隆株。从一只100日龄的雄性C3H/An小鼠的皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L,第95代的细胞系L使用毛细管法分离单细胞建立了NCTC clone 929 [L cell, L-929, derivative of Strain L-929]。 |
年龄(性别) | 100D Male |
组织来源 | 未知 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunosuppressed mice. |
STR位点信息
STR鉴定图
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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