HCC70(人乳腺导管癌细胞)
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货号: CL-0822
Price: ¥2500
Size:
1×10^6Cells/T25
产品信息
产品名称 | HCC70(人乳腺导管癌细胞) |
别称 | HCC-70;HCC 70;HCC0070;Hamon Cancer Center 70 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640+10% FBS+1% P/S 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) | 培养条件: 温度:37℃ |
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2-3次/周 |
注意事项 |
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参考资料(来源文献)
背景描述 | HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. |
年龄(性别) | 49Y Female |
组织来源 | 乳腺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺导管癌 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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