MTT溶液(5 mg/mL)
货号: PB180519
Price: ¥160 ¥240
产品信息
产品简介 | MTT全称为3-(4,5-dimethyl-2-thiazolyl)-2,5-dipHenyl-2-H-tetrazolium bromide,汉语化学名为 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐,商品名:噻唑蓝,是一种黄颜色的染料。MTT能够检测细胞存活和生长,其检测原理为:活细胞线粒体中的琥珀酸脱氢酶,能使外源性MTT还原为不溶于水的蓝紫色结晶物--甲瓒(Formazan),并沉积在细胞中,而死细胞则无此功能;二甲基亚砜(DMSO)能溶解细胞中的甲瓒,用酶标仪在490nm或570nm波长处(常用490nm)测定其光吸收值(OD值),通过OD值的大小,可以判断活细胞的数量或活性,在一定细胞数范围内,MTT结晶形成的量与活细胞的数量或活性成正比。该方法的特点是灵敏度高,并且十分经济,已广泛应用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。 |
产品形态 | 液体 |
产品浓度 | 5mg/mL |
产品规格 | 1mL×5 / 1mL×10 |
PH | 7.2-7.6 |
溶剂 | 10 mM PBS |
内毒素 | <3 |
储存条件 | -5~-20℃,避光储存 |
运输条件 | 低温 |
有效期 | 18个月 |
注意事项 |
|
-
Matrine protects colon mucosal epithelial cells against inflammation and apoptosis via the Janus kinase 2 /signal transducer and activator of transcription 3 pathway(2022)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2031676
影响因子:4.900
引用产品: MTT溶液 (5mg/mL)
-
Pigment epithelium-derived factor modulates periodontal homeostasis in mice and induces osteogenic differentiation of human periodontal ligament fibroblasts(2022)
期刊:Connective Tissue Research
DOI:10.1080/03008207.2021.2025224
影响因子:2.900
引用产品: MTT溶液 (5mg/mL)
-
Liquiritin reduces lipopolysaccharide-aroused HaCaT cell inflammation damage via regulation of microRNA-31/MyD88(2021)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2021.108283
影响因子:4.932
引用产品: MEM(含NEAA) 培养基, MTT溶液(5 mg/mL), Hacat 细胞, 特级胎牛血清
-
Expression of lncRNA CCAT2 in children with neuroblastoma and its effect on cancer cell growth(2021)
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-020-04042-9
影响因子:4.300
引用产品: MTT溶液 (5mg/mL)
-
Downregulation of MIAT reduces the proliferation and migratory and invasive abilities of retinoblastoma cells by sponging miR?665 and regulating LASP1(2021)
期刊:Experimental and Therapeutic Medicine
影响因子:2.700
引用产品: MTT溶液 (5mg/mL)
-
Rod-shape inorganic biomimetic mutual-reinforcing MnO2-Au nanozymes for catalysis-enhanced hypoxic tumor therapy(2020)
期刊:Nano Research
影响因子:9.900
引用产品: MTT溶液 (5mg/mL)
-
N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW(2020)
期刊:Frontiers in Cellular and Infection Microbiology
影响因子:5.500
引用产品: MTT溶液 (5mg/mL)
-
Mechanism of FGF7 gene silencing in regulating viability, apoptosis, invasion of retinoblastoma cell line HXO-Rb44 and angiogenesis.(2020)
期刊:European Review for Medical and Pharmacological Sciences
DOI:10.26355/eurrev_202004_20814
影响因子:3.800
引用产品: MTT溶液 (5mg/mL)
-
Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway(2020)
期刊:ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
影响因子:1.238
引用产品: MTT溶液(5 mg/mL)
-
Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells(2018)
期刊:Molecules
影响因子:4.600
引用产品: DMEM高糖 培养基, 磷酸盐缓冲液 (PBS) 1×, MTT溶液 (5mg/mL)

识别码示意图