MTT溶液(5 mg/mL)
货号: PB180519
Price: ¥160 ¥240
产品信息
产品简介 | MTT全称为3-(4,5-dimethyl-2-thiazolyl)-2,5-dipHenyl-2-H-tetrazolium bromide,汉语化学名为 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐,商品名:噻唑蓝,是一种黄颜色的染料。MTT能够检测细胞存活和生长,其检测原理为:活细胞线粒体中的琥珀酸脱氢酶,能使外源性MTT还原为不溶于水的蓝紫色结晶物--甲瓒(Formazan),并沉积在细胞中,而死细胞则无此功能;二甲基亚砜(DMSO)能溶解细胞中的甲瓒,用酶标仪在490nm或570nm波长处(常用490nm)测定其光吸收值(OD值),通过OD值的大小,可以判断活细胞的数量或活性,在一定细胞数范围内,MTT结晶形成的量与活细胞的数量或活性成正比。该方法的特点是灵敏度高,并且十分经济,已广泛应用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。 |
产品形态 | 液体 |
产品浓度 | 5mg/mL |
产品规格 | 1mL×5 / 1mL×10 |
PH | 7.2-7.6 |
溶剂 | 10 mM PBS |
内毒素 | <3 |
储存条件 | -5~-20℃,避光储存 |
运输条件 | 低温 |
有效期 | 18个月 |
注意事项 |
|
-
SERPINB7 as a prognostic biomarker in cervical cancer: Association with immune infiltration and facilitation of the malignant phenotype(2023)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e20184
影响因子:4.000
引用产品: MTT溶液(5 mg/mL), MEM 培养基, 特级胎牛血清, Hela 细胞
-
Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription(2023)
期刊:KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子:3.300
引用产品: MTT溶液 (5mg/mL)
-
Sfrp2 regulates the WNT/β-catenin pathway to slow the development of aldosterone-producing adenoma(2023)
期刊:Cardiovascular Diagnosis and Therapy
影响因子:2.400
引用产品: MTT溶液(5 mg/mL)
-
LncRNA FOXD3-AS1/miR-128-3p axis-mediated IGF2BP3 in glioma stimulates cancer angiogenesis and progression(2023)
期刊:FOLIA NEUROPATHOLOGICA
影响因子:2.000
引用产品: MTT溶液 (5mg/mL)
-
Kinetically and thermodynamically controlled one-pot growth of gold nanoshells with NIR-II absorption for multimodal imaging-guided photothermal therapy(2023)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-01907-1
影响因子:10.200
引用产品: 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×, 磷酸盐缓冲液 (PBS) 1×, MTT溶液 (5mg/mL), RPMI-1640 培养基
-
Identification of Circular RNA Circ_0003256 as a Novel Player in Pediatric Acute Myeloid Leukemia(2023)
期刊:JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY
DOI:10.1097/MPH.0000000000002372
影响因子:1.200
引用产品: MTT溶液 (5mg/mL)
-
BHLHE40 promotes osteoclastogenesis and abnormal bone resorption via c-Fos/NFATc1(2022)
期刊:Cell and Bioscience
DOI:10.1186/s13578-022-00813-7
影响因子:9.584
引用产品: MTT溶液(5 mg/mL)
-
Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata)(2022)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2022.1006316
影响因子:6.100
引用产品: DMSO, RPMI-1640 培养基, 特级胎牛血清, MTT溶液 (5mg/mL), B16-F10 细胞
-
Design, synthesis and insecticidal activity and mechanism research of Chasmanthinine derivatives(2022)
期刊:Scientific Reports
DOI:10.1038/s41598-022-19523-8
影响因子:5.000
引用产品: Sf9 细胞, MTT溶液 (5mg/mL), DMSO
-
Long non-coding RNA TTTY15 silencing inhibits gastric cancer progression by sponging microRNA-98-5p to down-regulate cyclin D2 expression(2022)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2047398
影响因子:4.900
引用产品: MTT溶液 (5mg/mL)

识别码示意图