货号: CL-0016
Price: ¥1400 ¥1400
生长培养基: Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A549 [A-549](人非小细胞肺癌细胞) |
别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系,源自于一位58岁白人男性;A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
年龄(性别) | 58Y Male |
组织来源 | 肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
-
Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction(2021)
作者:Hua Zhang, Yajuan Zhou, Dan Luo, Jingjian Liu
期刊:RSC Advances
影响因子:3.9
引用产品: BEAS-2B, A549 [A-549] 细胞
-
TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT(2021)
作者:Danjie Zhang, Liangzhang Sun, Jin Zhang
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2021.115793
影响因子:3.8
引用产品: BEAS-2B, A549 [A-549] 细胞, NCI-H226 [H226] 细胞, NCI-H520 细胞, NCI-H1975 细胞
-
Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer(2021)
作者:Kui Xiao, Yang Wang, Lihua Zhou
期刊:PLoS One
DOI:10.1371/journal.pone.0259091
影响因子:3.7
引用产品: BEAS-2B, A549 [A-549] 细胞
-
CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway(2021)
作者:Decai Chi, Dong Wang, Minghui Zhang
期刊:Experimental Cell Research
DOI:10.1016/j.yexcr.2021.112914
影响因子:3.7
引用产品: A549 [A-549] 细胞, HCC827 细胞, SK-MES-1 细胞
-
Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis(2021)
作者:Rui Zhang, Liang Shang, Jinniang Nan
期刊:Autoimmunity
DOI:10.1080/08916934.2021.2001801
影响因子:3.5
引用产品: T84 细胞, NCI-H1650 细胞, NCI-H1299 细胞, A549 [A-549] 细胞, RPMI-1640 培养基, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×
-
CircRNA circ_0008037 facilitates tumor growth and the Warburg effect via upregulating NUCKS1 by binding to miR-433-3p in non-small cell lung cancer(2021)
作者:Jia Yu, Haining Zhang, Chunsheng Zhao, Guanghui Li, Yingying Zhang, Yang Sun
期刊:Thoracic Cancer
影响因子:3.5
引用产品: A549 [A-549] 细胞, NCI-H1975 细胞, BEAS-2B 细胞
-
Ginsenoside CK Inhibits TGF-β-Induced Epithelial-Mesenchymal Transition in A549 Cell via SIRT1(2021)
作者:Mingyang Sun, Xuefeng Zhuang, Guangfu Lv
期刊:Biomed Research International
影响因子:3.3
引用产品: A549 [A-549] 细胞
-
Nuclear factor-kappaB regulates the transcription of NADPH oxidase 1 in human alveolar epithelial cells(2021)
作者:Weijing Wu, Li Li, Xiaoshan Su
期刊:BMC Pulmonary Medicine
DOI:10.1186/s12890-021-01464-z
影响因子:3.3
引用产品: A549 [A-549] 细胞
-
miR-324-3p reverses cisplatin resistance by inducing GPX4-mediated ferroptosis in lung adenocarcinoma cell line A549(2021)
作者:Shihua Deng, Dongming Wu, Li Li
期刊:Biochemical and Biophysical Research Communications
DOI:10.1016/j.bbrc.2021.02.077
影响因子:3.1
引用产品: A549 [A-549] 细胞, A549/DDP 细胞
-
Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis(2021)
作者:Dan Chu, Pengpeng Li, Yameng Li
期刊:Thoracic Cancer
影响因子:2.9
引用产品: 人支气管上皮细胞, A549 [A-549] 细胞, A549/DDP 细胞, NCI-H1299 细胞
-
Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

识别码示意图