货号: CL-0016
Price: ¥1400 ¥1400
生长培养基: Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | A549 [A-549](人非小细胞肺癌细胞) |
别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系,源自于一位58岁白人男性;A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
年龄(性别) | 58Y Male |
组织来源 | 肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
-
Characterization of fatty acid metabolism-related lncRNAs in lung adenocarcinoma identifying potential novel prognostic targets(2022)
作者:Liu Yang, Zhang Xingshu, Cheng Xuechao, Luo Qian, Yu Mingyang, Long Kaijun, Qu Wendong, Tang Yang, Gong Ming, Liang Lubiao, Ke Xixian, Song Yongxiang
期刊:Frontiers in Genetics
影响因子:4.8
引用产品: NCI-H1299 细胞, BEAS-2B 细胞, A549 [A-549] 细胞
-
In vivo fluorescent screening for HPPD-targeted herbicide discovery(2022)
作者:Yi-Xuan Fu, Zi-Ye Zhang, Wu-Yingzheng Guo, Yi-Jie Dai, Zheng-Yu Wang, Wen-Chao Yang, Guang-Fu Yang
期刊:PEST MANAGEMENT SCIENCE
DOI:10.1002/ps.7117
影响因子:4.5
-
IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway(2022)
作者:Ruzhen Li, Zhaohui Han, Wei Ma, Lin Zhang, Xiangwei Zhang, Yuanzhu Jiang, Wei Dong
期刊:Journal of Oncology
影响因子:4.5
引用产品: NCI-H1975 细胞, NCI-H1299 细胞, A549 [A-549] 细胞, NCI-H23 细胞, 16HBE 细胞
-
Long noncoding RNA OIP5-AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability(2022)
作者:Chengye Mao, Xionghui Li
期刊:ENVIRONMENTAL TOXICOLOGY
影响因子:4.5
引用产品: BEAS-2B, NCI-H1299 细胞, A549 [A-549] 细胞, NCI-H1299细胞专用培养基, A549 [A-549]细胞专用培养基, BEAS-2B细胞专用培养基, 293T [HEK-293T] 细胞, NCI-H1975 细胞, NCI-H596 细胞
-
DNA repair/recombination protein 54L promotes the progression of lung adenocarcinoma by activating mTORC1 pathway(2022)
作者:Liu Changjiang, Ren Wei, Zhang Zhixin, Guan Juanjuan
期刊:Human Cell
DOI:10.1007/s13577-022-00832-z
影响因子:4.4
引用产品: NCI-H1299 细胞, NCI-H23 细胞, NCI-H1975 细胞, 16HBE 细胞, A549 [A-549] 细胞
-
Circ_0025039 acts an oncogenic role in the progression of non-small cell lung cancer through miR-636-dependent regulation of CORO1C(2022)
作者:Lei Wang, Cimei Zeng, Zhongren Chen
期刊:Molecular and Cellular Biochemistry
DOI:10.1007/s11010-021-04320-0
影响因子:4.3
引用产品: 16HBE 细胞, A549 [A-549] 细胞, NCI-H1299 细胞, RPMI-1640 培养基, Ham's F-12K 培养基, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×, PUMC-HUVEC-T1
-
Ratiometric fluorescence and colorimetric dual-mode sensing platform based on carbon dots for detecting copper(II) ions and D-penicillamine(2022)
作者:Wei Zhang, Yu Zhang, Xin Liu
期刊:ANALYTICAL AND BIOANALYTICAL CHEMISTRY
DOI:10.1007/s00216-021-03789-4
影响因子:4.3
引用产品: A549 [A-549] 细胞
-
High-content screening of active components of Traditional Chinese Medicine inhibiting TGF-β-induced cell EMT(2022)
作者:Mengzhen Xu, Qinghua Cui, Wen Su, Dan Zhang, Jiaxu Pan, Xiangqi Liu, Zheng Pang, Qingjun Zhu
期刊:Heliyon
DOI:10.1016/j.heliyon.2022.e10238
影响因子:3.8
引用产品: A549 [A-549] 细胞
-
Tanshinone IIA (TSIIA) represses the progression of non-small cell lung cancer by the circ_0020123/miR-1299/HMGB3 pathway(2022)
作者:Sun Fei, Yang Xiaoli, Song Wei, Yu Nan, Lin Qingyi
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-022-04646-3
影响因子:3.8
引用产品: A549 [A-549] 细胞, NCI-H292 细胞, Ham's F-12K 培养基, RPMI-1640 培养基, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×
-
SLC7A5 is a lung adenocarcinoma-specific prognostic biomarker and participates in forming immunosuppressive tumor microenvironment(2022)
作者:Yong Liu, Guoyuan Ma, Jichang Liu, Haotian Zheng, Gemu Huang, Qingtao Song, Zhaofei Pang, Jiajun Du
期刊:Heliyon
DOI:10.1016/j.heliyon.2022.e10866
影响因子:3.8
引用产品: A549 [A-549] 细胞, HCC827 细胞, NCI-H1975 细胞, PC-9 细胞
-
Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

识别码示意图