货号： CL-0016
Price： ￥1400 ￥1400
生长培养基： Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | A549 [A-549]（人非小细胞肺癌细胞） |
| 别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:Ham's F-12K[PM150910]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系，源自于一位58岁白人男性；A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
| 年龄（性别） | 58Y Male |
| 组织来源 | 肺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Chelating drug-induced labile Zn2+ with nanoparticle-encapsulated TPEN at low dose enhances lung cancer chemotherapy through inhibiting ABCB1(2024)
作者：Linlin Wang, Chen Ni, Kaili Zhang, Yuanyuan Yang, Ruoyang Chen, Xiaohan Lou, Yan Yan, Kexin Li, Ya Dong, Xiaohan Yao, Jiajia Wan, Xixi Duan, Fazhan Wang, YongJuan Li, Zhihai Qin
期刊：iScience
DOI：10.1016/j.isci.2024.111072
影响因子：4.6
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Cytotoxic diterpenoids from Croton kongensis inhibiting tumor proliferation and migration(2024)
作者：Qiong Zhu, Ying Li, Chenglei Wang, Jinhai Yu, Jianmin Yue, Bin Zhou
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2024.107739
影响因子：4.5
引用产品： MDA-MB-231 细胞, MCF7 [MCF-7] 细胞, A549 [A-549] 细胞, NCI-H1975 细胞, Hela 细胞
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Citrulline facilitates the glycolysis, proliferation, and metastasis of lung cancer cells by regulating RAB3C(2024)
作者：Qingjun Meng, Yanguang Li, Zhen Sun, Junfeng Liu
期刊：ENVIRONMENTAL TOXICOLOGY
影响因子：4.5
引用产品： NCI-H1299 细胞, A549 [A-549] 细胞
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Macrophage-Related Gene Signatures for Predicting Prognosis and Immunotherapy of Lung Adenocarcinoma by Machine Learning and Bioinformatics(2024)
作者：Yunzhi Xiang, Guanghui Wang, Baoliang Liu, Haotian Zheng, Qiang Liu, Guoyuan Ma, Jiajun Du
期刊：Journal of Inflammation Research
影响因子：4.5
引用产品： A549 [A-549] 细胞, PC-9 细胞
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METTL3-Dependent YTHDF2 Mediates TSC1 Expression to Regulate Alveolar Epithelial Mesenchymal Transition and Promote Idiopathic Pulmonary Fibrosis(2024)
作者：Min Liu, Yingying Sheng, Mengyu Li, Tianyu Pan, Wei Jiang, Yafei Zhang, Xin Pan, Cheng Huang, Jun Li, Yuanyuan Wang
期刊：JOURNAL OF CELLULAR PHYSIOLOGY
影响因子：4.5
引用产品： A549 [A-549] 细胞
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HNRNPD/MAD2L2 axis facilitates lung adenocarcinoma progression and is a potential prognostic biomarker(2024)
作者：Zhuoyu Gu, Weizheng Ding, Shuang Yuan, Youqiang Peng, Bo Dong, Yike Gu, Jing Li, Yitong Chen, Kailu Wang, Tianze Liu, Xiaodan Han, Yixin Li
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2024.111443
影响因子：4.4
引用产品： Calu-3 细胞, A549 [A-549] 细胞, NCI-H1975 细胞, PC-9 细胞, 人脐静脉内皮细胞
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Epigenetic Regulation of RNF135 by LSD1 Promotes Stemness Maintenance and Brain Metastasis in Lung?Adenocarcinoma(2024)
作者：Xiaohan Qu, Tianjian Ding, Haoqi Zhao, Liming Wang
期刊：ENVIRONMENTAL TOXICOLOGY
影响因子：4.4
引用产品： Ham's F-12K 培养基, A549 [A-549] 细胞, RPMI-1640 培养基, NCI-H1975 细胞
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GALNT14-mediated O-glycosylation drives lung adenocarcinoma progression by reducing endogenous reactive oxygen species generation(2024)
作者：Bingbing Tang, Kelong Wang, Qiulei Ren, Junshuo Zhou, Yuewen Xu, Liaoyuan Liu, Bin Yin, Yaling Zhang, Qian Huang, Ruiqi Lv, Zhiguo Luo, Hongyan Zhao, Li Shen
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2024.111477
影响因子：4.4
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Triiodothyronine acts on DAO to regulate pulmonary fibrosis progression by facilitating cell senescence through the p53/p21 signaling pathway(2024)
作者：Guo Xiaoshu, Xu Kai, Wang Lan, Ding Linke, Li Wenwen, Zhang Xinsheng, Zhao Weiming, Wang Ningdan, Wang Gaiping, Zhao Wenyu, Rosas Ivan, Yu Guoying
期刊：Frontiers in Pharmacology
DOI：10.3389/fphar.2024.1433186
影响因子：4.4
引用产品： A549 [A-549] 细胞
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Effect of the combined binding of topotecan and catechin/protocatechuic acid to a pH-sensitive DNA tetrahedron on release and cytotoxicity: Spectroscopic and calorimetric studies(2024)
作者：Lu Wang, Jie Liu, Xiangtai Wang, Xinyu Li, Xinpeng Zhang, Lixia Yuan, Yushu Wu, Min Liu
期刊：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
影响因子：4.4
引用产品： A549 [A-549] 细胞, HL-7702 [L-02; LO2] 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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