货号: CL-0166
Price: ¥1600 ¥1600
生长培养基: RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | NCI-H1650(人非小细胞肺癌细胞) |
别称 | H1650; H-1650; H1650_CO; NCIH1650 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | NCI-H1650细胞是于1987年从一名27岁白人男性(10年烟龄)支气管肺泡癌患者的胸腔积液中分离得到的。 |
年龄(性别) | 27Y Male |
组织来源 | 肺;腺癌;细支气管及肺泡癌;转移灶:胸水 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Super-Resolution Fluorescence Imaging Reveals the Mechanism of NRP1 Clustering on Non-Small-Cell Lung Cancer Membranes(2025)
作者:Meng Li, Jing Gao, Xiao Qi, Baofeng Li, Yinghao Zhao, Xiangyu Liu, Guangxin Zhang, Hongda Wang, Ti Tong
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.4c05675
影响因子:6.7
引用产品: NCI-H1650 细胞, NCI-H1975 细胞, HCC827 细胞
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Brain Metastasis from EGFR-Mutated Non-Small Cell Lung Cancer: Secretion of IL11 from Astrocytes Up-Regulates PDL1 and Promotes Immune Escape(2024)
作者:Mengyi Tang, Mingxin Xu, Jian Wang, Ye Liu, Kun Liang, Yinuo Jin, Wenzhe Duan, Shengkai Xia, Guohui Li, Huiying Chu, Wenwen Liu, Qi Wang
期刊:Advanced Science
影响因子:15.1
引用产品: LLC [LL/2; LLC1] 细胞, NCI-H1650 细胞
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N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing(2024)
作者:Wang Anqi, Zeng Yuanyuan, Zhang Weijie, Zhao Jian, Gao Lirong, Li Jianjun, Zhu Jianjie, Liu Zeyi, Huang Jian-an
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00622-5
影响因子:9.2
引用产品: BEAS-2B 细胞, NCI-H226 [H226] 细胞, HCC827 细胞, NCI-H1299 细胞, RPMI-1640 培养基 , A549 [A-549] 细胞, PC-9 细胞, DMEM高糖 培养基 , 293T [HEK-293T] 细胞, NCI-H1650 细胞, NCI-H1975 细胞
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NOP2 facilitates EZH2-mediated epithelial–mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression(2024)
作者:Yang Ying, Fan Hongzhao, Liu Hongyang, Lou Xueling, Xiao Nan, Zhang Chenxing, Chen Huanxiang, Chen Shuangshuang, Gu Huihui, Liu Hongchun, Wan Junhu
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06899-w
影响因子:8.1
引用产品: A549 [A-549] 细胞, NCI-H358 细胞, NCI-H1650 细胞, NCI-H1299 细胞
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ORC6 acts as an effective prognostic predictor for non?small cell lung cancer and is closely associated with tumor progression(2024)
作者:Letian Chen, Dongdong Zhang, Yujuan Chen, Huilan Zhu, Zhipeng Liu, Zhiping Yu, Junping Xie
期刊:Oncology Letters
影响因子:2.9
引用产品: A549 [A-549] 细胞, PC-9 细胞, NCI-H1299 细胞, NCI-H1650 细胞, BEAS-2B 细胞
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Identification of a necroptosis-related gene signature for making clinical predictions of the survival of patients with lung adenocarcinoma(2024)
作者:Xiaoping Zhou, Ming Zhao, Yingzi Fan, Ying Xu
期刊:PeerJ
影响因子:2.7
引用产品: BEAS-2B 细胞, A549 [A-549] 细胞, NCI-H1650 细胞
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Curcumin Inhibits the Progression of Non-small Cell Lung Cancer by Regulating DMRT3/SLC7A11 Axis(2024)
作者:Xu Bin, Zhou Li, Zhang Qian
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01166-x
影响因子:2.6
引用产品: 人脐静脉内皮细胞, 16HBE 细胞, NCI-H1650 细胞
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USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer(2024)
作者:Wencui Kong, Xuegang Feng, Zongyang Yu, Xingfeng Qi, Zhongquan Zhao
期刊:Thoracic Cancer
影响因子:2.3
引用产品: NCI-H1299 细胞, THP-1 细胞, HBE 细胞, A549 [A-549] 细胞, HCC827 细胞, NCI-H1650 细胞
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MECOM: a bioinformatics and experimentally identified marker for the diagnosis and prognosis of lung adenocarcinoma(2024)
作者:Anqi Li, Meng Li, Jing Wang, Jiejun Zhou, Tian Yang, Meng Fan, Kun Zhang, Hengxing Gao, Hui Ren, Mingwei Chen
期刊:Biomarkers in Medicine
影响因子:1.9
引用产品: A549 [A-549] 细胞, NCI-H1650 细胞, BEAS-2B 细胞
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circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity(2024)
作者:Wang Chun, Xu Bin, Tao Chengzhi, Lin Huan, Liu Dan, Zhang Haitao
期刊:Journal of Cardiothoracic Surgery
DOI:10.1186/s13019-024-02964-9
影响因子:1.5
引用产品: BEAS-2B 细胞, NCI-H1650 细胞, Calu-3 细胞, A549 [A-549] 细胞, NCI-H1299 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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