货号: CL-0165
Price: ¥1600 ¥1600
生长培养基: RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | NCI-H1299(人非小细胞肺癌细胞) |
别称 | H1299; H-1299; NCIH1299 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | NCI-H1299细胞来源于一个淋巴结转移,患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白,并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1 pmol/毫克蛋白的NMB蛋白,而不合成促胃液释放肽(GRP)。 |
年龄(性别) | 43Y Male |
组织来源 | 肺癌:非小细胞肺癌;转移灶:淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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Cinobufagin disrupts the stability of lipid rafts by inhibiting the expression of caveolin-1 to promote non-small cell lung cancer cell apoptosis(2024)
作者:Zhongqing Xu, Jinwei Li, Shuyu Fang, Mingzhu Lian, Changxiao Zhang, Jiahuan Lu, Kai Sheng
期刊:Archives of Medical Science
影响因子:3.0
引用产品: 16HBE 细胞, A549 [A-549] 细胞, NCI-H1299 细胞
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IGF2BP3 loss inhibits cell progression by upregulating has_circRNA_103820, and hsa_circRNA_103820-encoded peptide inhibits cell progression by inactivating the AKT pathway in lung cancer(2024)
作者:Jinrong Zhou, Lixia Yao, Yuan Su, Lili Tian
期刊:Chemical Biology & Drug Design
影响因子:3.0
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ORC6 acts as an effective prognostic predictor for non?small cell lung cancer and is closely associated with tumor progression(2024)
作者:Letian Chen, Dongdong Zhang, Yujuan Chen, Huilan Zhu, Zhipeng Liu, Zhiping Yu, Junping Xie
期刊:Oncology Letters
影响因子:2.9
引用产品: A549 [A-549] 细胞, PC-9 细胞, NCI-H1299 细胞, NCI-H1650 细胞, BEAS-2B 细胞
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METTL3-mediated the m6A modification of SF3B4 facilitates the development of non-small cell lung cancer by enhancing LSM4 expression(2024)
作者:Guangsi He, Kangsheng Gu, Jie Wei, Jian Zhang
期刊:Thoracic Cancer
影响因子:2.9
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞, 16HBE 细胞
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Unlocking the Potential of Disulfidptosis-Related LncRNAs in Lung Adenocarcinoma: A Promising Prognostic LncRNA Model for Survival and Immunotherapy Prediction(2024)
作者:Xin Nie, Hong Ge, Kongming Wu, Ru Liu, Chunyu He
期刊:Cancer Medicine
影响因子:2.9
引用产品: NCI-H1299 细胞, BEAS-2B 细胞, NCI-H460 [H460] 细胞, NCI-H1975 细胞
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Curcumin suppresses copper accumulation in non-small cell lung cancer by binding ATOX1(2024)
作者:Qin Xiao, Wang Peng, Liang Haiyue, Si Wentao
期刊:BMC Pharmacology & Toxicology
DOI:10.1186/s40360-024-00784-0
影响因子:2.8
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞
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Poor therapeutic outcomes in KRAS-mutant non-small cell lung cancer due to chemoresistance conferred by SLC7A11(2024)
作者:Zhang Shiyu, Ge Yutong, Liu Jingwen, Lu Kaihua
期刊:Clinical & Translational Oncology
DOI:10.1007/s12094-024-03592-4
影响因子:2.8
引用产品: A549 [A-549] 细胞, NCI-H1975 细胞, NCI-H23 细胞, NCI-H1299 细胞
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Ampelopsis japonica enhances the effect of radiotherapy in non-small cell lung cancer(2024)
作者:Liu Zhaohua, Cui Peixia, Wu Qian, Ji Xiao
期刊:STRAHLENTHERAPIE UND ONKOLOGIE
DOI:10.1007/s00066-024-02322-7
影响因子:2.7
引用产品: HCC827 细胞, NCI-H1299 细胞, 青霉素-链霉素溶液(双抗),100×
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High core 1β1,3-galactosyltransferase 1 expression is associated with poor prognosis and promotes cellular radioresistance in lung adenocarcinoma(2024)
作者:Chen Yong, Ji Yanyan, Shen Lin, Li Ying, Ren Yue, Shi Hongcan, Li Yue, Wu Yunjiang
期刊:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI:10.1007/s00432-024-05745-y
影响因子:2.7
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞
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POU4F1 enhances lung cancer gemcitabine resistance by regulating METTL3-dependent TWF1 mRNA N6 adenosine methylation(2024)
作者:Tang Jianfeng, Liu Zhijian, Xie Guanghui, Wang Chenbin, Jiang Yongjun
期刊:3 Biotech
DOI:10.1007/s13205-024-04161-w
影响因子:2.6
引用产品: A549 [A-549] 细胞, NCI-H1299 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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