货号: CL-0298
Price: ¥2000 ¥2000
生长培养基: RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | NCI-H1975(人肺腺癌细胞) |
别称 | H1975; H-1975 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[PM150110]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
注意事项 |
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参考资料(来源文献)
背景描述 | NCI-H1975细胞是于1988年7月从一名女性(无抽烟史)非小细胞肺腺癌组织中分离得到的。 |
年龄(性别) | Age unspecified Female |
组织来源 | 器官:肺;疾病:腺癌;非小细胞肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma(2021)
作者:Xiaoxia Dong, Yongyu Liu, Xinzhou Deng
期刊:Frontiers in Cell and Developmental Biology
影响因子:5.5
引用产品: BEAS-2B, A549 [A-549] 细胞, NCI-H1299 细胞, NCI-H1975 细胞, NCI-H441 细胞
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Inhibition effect of oxyepiberberine isolated from Coptis chinensis franch. On non-small cell lung cancer based on a network pharmacology approach and experimental validation(2021)
作者:Ying Zhang, Yunfeng Yao, Yingjie Fu
期刊:Journal Of Ethnopharmacology
影响因子:5.4
引用产品: BEAS-2B, A549 [A-549] 细胞, NCI-H1975 细胞
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Exosomal Delivery of FTO Confers Gefitinib Resistance to Recipient Cells through ABCC10 Regulation in an m6A-dependent Manner(2021)
作者:Peng Xiao, Yukang Liu, Wei Han
期刊:Molecular Cancer Research
DOI:10.1158/1541-7786.MCR-20-0541
影响因子:5.2
引用产品: PC-9 细胞, NCI-H1975 细胞
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TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT(2021)
作者:Danjie Zhang, Liangzhang Sun, Jin Zhang
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2021.115793
影响因子:3.8
引用产品: BEAS-2B, A549 [A-549] 细胞, NCI-H226 [H226] 细胞, NCI-H520 细胞, NCI-H1975 细胞
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RNA editing enzyme adenosine deaminases acting on RNA 1 deficiency increases the sensitivity of non-small cell lung cancer cells to anlotinib by regulating CX3CR1-fractalkine expression(2021)
作者:Min Wu, Mengmeng Jin, Xiaohui Cao
期刊:Drug Development Research
影响因子:3.8
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Comprehensive analysis of an immune infiltrate-related competitive endogenous RNA network reveals potential prognostic biomarkers for non-small cell lung cancer(2021)
作者:Caizhi Yang, Leihao Hu, Zhongyu Huang
期刊:PLoS One
DOI:10.1371/journal.pone.0260720
影响因子:3.7
引用产品: 青霉素-链霉素溶液 (双抗),100×, NCI-H1650 细胞, NCI-H1975 细胞, DMEM高糖 培养基
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CircRNA circ_0008037 facilitates tumor growth and the Warburg effect via upregulating NUCKS1 by binding to miR-433-3p in non-small cell lung cancer(2021)
作者:Jia Yu, Haining Zhang, Chunsheng Zhao, Guanghui Li, Yingying Zhang, Yang Sun
期刊:Thoracic Cancer
影响因子:3.5
引用产品: A549 [A-549] 细胞, NCI-H1975 细胞, BEAS-2B 细胞
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Calponin 3 suppresses proliferation, migration and invasion of non?small cell lung cancer cells(2021)
作者:Chenglin Yang, Shiping Zhu, Weifeng Feng
期刊:Oncology Letters
影响因子:2.9
引用产品: PC-9 细胞, NCI-H1975 细胞, NCI-H520 细胞
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Long noncoding RNA LINC00968 inhibits proliferation, migration and invasion of lung adenocarcinoma through targeting miR-22-5p/CDC14A axis(2021)
作者:Chao Wu, Xuzhao Bian, Liyuan Zhang
期刊:3 BIOTECH
DOI:10.1007/s13205-021-02981-8
影响因子:2.9
引用产品: A549 [A-549] 细胞, NCI-H1975 细胞
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Anticancer roles of let?7f?1?3p in non?small cell lung cancer via direct targeting of integrin β1(2021)
作者:Yanan Yang, Yuanrong Liu,Ning Xie
期刊:Experimental and Therapeutic Medicine
影响因子:2.7
引用产品: NCI-H1975 细胞
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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