货号: CL-0525
Price: ¥2000 ¥2000
生长培养基: DMEM/F12[PM150312]+5% HS[164215]+20 ng/mL EGF[PCK001]+0.5 μg/mL Hydrocortisone+10 μg/mL Insulin[PB180432]+1% NEAA[PB180424]+1% P/S[PB180120]
产品信息
产品名称 | MCF 10A(人正常乳腺上皮细胞) |
别称 | MCF 10A; MCF.10A; MCF10A; MCF10a; MCF-10 Attached; Michigan Cancer Foundation-10A |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:DMEM/F12[PM150312]+5% HS[164215]+20 ng/mL EGF[PCK001]+0.5 μg/mL Hydrocortisone+10 μg/mL Insulin[PB180432]+1% NEAA[PB180424]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 |
|
参考资料(来源文献)
年龄(性别) | 36Y Female |
组织来源 | 乳腺;乳房 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
STR位点信息
STR鉴定图
-
Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3(2023)
作者:Xue Hu, Baoliang Guo, Tong Sun, Wan Wang
期刊:Open Medicine
影响因子:2.1
引用产品: MCF 10A 细胞, MCF7 [MCF-7] 细胞, MDA-MB-231 细胞, MDA-MB-436 细胞, SK-BR-3 [SKBR3] 细胞, DMEM/F12 培养基, 马血清, MEM非必需氨基酸溶液 (NEAA),100×, 青霉素-链霉素溶液 (双抗),100×, RPMI-1640 (ATCC改良) 培养基, Leibovitz's L-15 培养基, McCoy's 5A 培养基, 特级胎牛血清
-
FEZF2 inhibits the growth of triple-negative breast cancer cells through EZH2/PD-L1 and enhances anti-tumor immunity in vivo(2023)
作者:Li Wenyu, Liu Hu, Li Wenjuan, Zhang Qiujun, Zhang Qianyu, Hu Dandan
期刊:Molecular & Cellular Toxicology
DOI:10.1007/s13273-023-00368-9
影响因子:1.7
引用产品: MCF 10A 细胞, DMEM/F12 培养基, 马血清, 青霉素-链霉素溶液 (双抗),100×, MDA-MB-231 细胞, MDA-MB-468 细胞, DMEM高糖 培养基, BT-549 细胞, RPMI-1640 培养基, MDA-MB-436 细胞
-
In vitro study of piwi interaction RNA-31106 promoting breast carcinogenesis by regulating METTL3-mediated m6A RNA methylation(2023)
作者:Shengchao Huang, Baoying Chen, Pu Qiu, Zeming Yan, Zhongzeng Liang, Kangwei Luo, Baoyi Huang, Haiping Jiang
期刊:Translational Cancer Research
影响因子:0.9
引用产品: MDA-MB-231 细胞, MCF7 [MCF-7] 细胞, MCF 10A 细胞, DMEM高糖 培养基
-
Expression of LINC00461 in breast cancer cells and its modulatory effect on miR-607/SLCLA3 axis(2023)
作者:Dan Li, Shuangjian Li, Qian Zhao, Dilixiati Jinsihan, Jinchun Feng
期刊:TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
影响因子:0.6
引用产品: MCF 10A 细胞, MDA-MB-468 细胞, T-47D 细胞, MCF7 [MCF-7] 细胞, MDA-MB-231 细胞, DMEM/F12 培养基, 特级胎牛血清, 青霉素-链霉素溶液 (双抗),100×
-
Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection(2023)
作者:Kun Li, Chunzi Liang, Yuling Pan, Linghan Mei, Guo-Jun Zhang
期刊:STAR Protocols
DOI:10.1016/j.xpro.2023.102516
影响因子:
引用产品: MCF 10A 细胞, MCF 10A细胞专用培养基
-
Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription(2022)
作者:Weiwei Yang , Jing Li, Minghui Zhang
期刊:Journal Of Experimental & Clinical Cancer Research
DOI:10.1186/s13046-022-02260-1
影响因子:11.3
引用产品: Hs 578T 细胞, BT-549 细胞, HCT-15 细胞, MCF 10A 细胞, A549 [A-549] 细胞, SK-OV-3 细胞, Hela 细胞, PANC-1 细胞
-
Polydopamine-Engineered Theranostic Nanoscouts Enabling Intracellular HSP90 mRNAs Fluorescence Detection for Imaging-Guided Chemo–Photothermal Therapy(2022)
作者:Geng Yang, Mengyue Li, Ting Song, Xiangyan Chen, Hanxi Zhang, Xiaodan Wei, Ningxi Li, Tingting Li, Xiang Qin, Shun Li, Fengming You, Chunhui Wu, Wei Zhang, Yiyao Liu, Hong Yang
期刊:Advanced Healthcare Materials
影响因子:11.1
引用产品: MCF 10A 细胞
-
Mitochondrial Protein UCP1 Inhibits the Malignant Behaviors of Triple-negative Breast Cancer through Activation of Mitophagy and Pyroptosis(2022)
作者:Jing Xia, Changbin Chu, Wanqing Li
期刊:International Journal of Biological Sciences
影响因子:10.8
引用产品: MCF 10A 细胞, MCF 10A细胞专用培养基
-
Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity(2022)
作者:Sumei Ren, Xiaodong Wang, Guangyi Jin
期刊:International Journal of Biological Sciences
影响因子:9.2
引用产品: MCF 10A 细胞
-
Solasodine suppress MCF7 breast cancer stem-like cells via targeting Hedgehog/Gli1(2022)
作者:Jing Chen, Da Ma, Cuicui Zeng, Lorenzo V. White, Huanqing Zhang, Yinglai Teng, Ping Lan
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2022.154448
影响因子:6.7
引用产品: MCF7 [MCF-7] 细胞, MCF 10A细胞, MCF 10A 细胞
-
Q1:MCF 10A中的hydrocortisone(皮质醇,氢化可的松)如何配置使用?
需要用DMSO配制成1mg/mL的母液,然后使用培养基稀释到工作浓度后使用。
-
Q2:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

识别码示意图