货号: CL-0228
Price: ¥1600 ¥1600
生长培养基: RPMI-1640[PM150110]+10 μg/mL Insulin[PB180432]+10% FBS[164210]+1% P/S[PB180120]
产品信息
产品名称 | T-47D(人乳腺管癌细胞) |
别称 | T-47-D; T47-D; T47D:A; T47D |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:RPMI-1640[PM150110]+10 μg/mL Insulin[PB180432]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃ |
培养方案B(可选) |
生长培养基: 培养条件: 气相:, 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | T-47细胞分离自一位54岁乳房侵入性导管癌的女性患者胸水;发现分化的上皮亚株(T-47D)有细胞质连接和17-β-雌二醇及其他类固醇降血钙素的受体,T-47D细胞表达WNT7B癌基因。 |
年龄(性别) | 54Y Female |
组织来源 | 乳腺;乳房;导管;来自导管癌肋膜渗出液转移灶 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, forms colonies in soft agar. |
受体表达情况 | calcitonin, expressed; androgen receptor, expressed; estrogen receptor, expressed; progesterone receptor, expressed; glucocorticoid receptor, positive, expressed; prolactin, expressed; calcitonin; androgen receptor, positive; progesterone receptor, p |
STR位点信息
STR鉴定图
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Prognostic and immune correlation of IDO1 promoter methylation in breast cancer(2024)
作者:Ding Shirong, Yang Ruozhu, Meng Jiahao, Guan Xinyu, Hong Yue, Xu Jiachi, Qu Limeng, Ji Jingfen, Yi Wenjun, Zou Qiongyan, Long Qian
期刊:Scientific Reports
DOI:10.1038/s41598-024-79149-w
影响因子:3.8
引用产品: DMEM高糖 培养基, T-47D 细胞, JIMT-1 细胞, MDA-MB-231 细胞, SUM159PT 细胞, MEM完全培养基(含10% FBS), 特级胎牛血清, A549 [A-549] 细胞, MCF7 [MCF-7] 细胞, ZR-75-1 细胞, 青霉素-链霉素溶液(双抗),100×, RPMI-1640 培养基, HCC1806 细胞
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Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation(2024)
作者:Liu Li, Zhao Qingqing, Xiong Daigang, Li Dan, Du Jie, Huang Yunfei, Yang Yan, Chen Rui
期刊:Scientific Reports
DOI:10.1038/s41598-024-63427-8
影响因子:3.8
引用产品: MCF 10A 细胞, BT-474 [BT474] 细胞, BT-549 细胞, SK-BR-3 [SKBR3] 细胞, MDA-MB-231 细胞, MDA-MB-468-06 细胞, MDA-MB-436 细胞, MCF7 [MCF-7] 细胞, T-47D 细胞
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Non-apoptotic regulatory cell death scoring system to predict the clinical outcome and drug choices in breast cancer(2024)
作者:Qiwang Zhou, Xiaokang Gao, Hui Xu, Xuan Lu
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e31342
影响因子:3.4
引用产品: MCF 10A 细胞, T-47D 细胞
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Synthesis of a biocompatible fluorinated multiblock copolymer surfactant for water-in-fluorocarbon droplets and its application in droplet digital polymerase chain reaction(2024)
作者:Ruijie Feng, Zeqin Li, Junyan Zhu, Yayun Zhang, Hao Fang, Jinbing Xue, Jinxian Wang, Renliang Lyu, Gangyin Luo
期刊:JOURNAL OF APPLIED POLYMER SCIENCE
影响因子:3.0
引用产品: T-47D 细胞
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Kuwanon C inhibits proliferation and induction of apoptosis via the intrinsic pathway in MDA-MB231 and T47D breast cancer cells(2024)
作者:Peng Qian, Gangxiang Yuan, Chao Yang, Qi Zhang, Lin Chen, Ningjia He
期刊:STEROIDS
DOI:10.1016/j.steroids.2024.109450
影响因子:2.7
引用产品: T-47D 细胞, MDA-MB-231 细胞
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FOXM1 derived from Triple negative breast cancer exosomes promotes cancer progression by activating IDO1 transcription in macrophages to suppress ferroptosis and induce M2 polarization of Tumor-associated macrophages(2024)
作者:Tielin Wang, Yan Zhang, Hong Liu, Jian Wu
期刊:GENES & GENETIC SYSTEMS
影响因子:1.0
引用产品: MCF 10A 细胞, SK-BR-3 [SKBR3] 细胞, BT-474 [BT474] 细胞, T-47D 细胞, MCF7 [MCF-7] 细胞
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A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer(2023)
作者:Song Runjie, Guo Peilan, Ren Xin, Zhou Lijun, Li Peng, Rahman Nafis A, Wo czyński S awomir, Li Xiru, Zhang Yanjun, Liu Mei, Liu Jiali, Li Xiangdong
期刊:Molecular Cancer
DOI:10.1186/s12943-023-01806-x
影响因子:37.3
引用产品: MDA-MB-231 细胞, MDA-MB-468 细胞, 293T [HEK-293T] 细胞, Hs 578T 细胞, MCF7 [MCF-7] 细胞, T-47D 细胞, ZR-75-1 细胞, MCF 10A 细胞
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Novel Self-Assembled Multifunctional Nanoprobes for Second-Near-Infrared-Fluorescence-Image-Guided Breast Cancer Surgery and Enhanced Radiotherapy Efficacy(2023)
作者:Yong-Qu Zhang, Wan-Ling Liu, Xiang-Jie Luo, Jun-Peng Shi, Yun-Zhu Zeng, Wei-Ling Chen, Wen-He Huang, Yuan-Yuan Zhu, Wen-Liang Gao, Rong-Hui Li, Zi-He Ming, Li-Xin Zhang, Rui-Qin Yang, Jia-Zheng Wang, Guo-Jun Zhang
期刊:Advanced Science
影响因子:15.1
引用产品: MDA-MB-231 细胞, MDA-MB-231/GFP 细胞, MCF7 [MCF-7] 细胞, T-47D 细胞, MCF 10A 细胞
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Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging(2023)
作者:Zhu Yuan-Yuan, Song Liang, Zhang Yong-Qu, Liu Wan-Ling, Chen Wei-Ling, Gao Wen-Liang, Zhang Li-Xin, Wang Jia-Zheng, Ming Zi-He, Zhang Yun, Zhang Guo-Jun
期刊:CANCER RESEARCH
DOI:10.1158/0008-5472.CAN-22-3432
影响因子:11.2
引用产品: 4T1 细胞, MDA-MB-231 细胞, MCF7 [MCF-7] 细胞, BT-549 细胞, T-47D 细胞, SK-BR-3 [SKBR3] 细胞, BT-474 [BT474] 细胞, MCF 10A 细胞
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Galangin attenuates doxorubicin-induced cardiotoxicity via activating nuclear factor erythroid 2-related factor 2/heme oxygenase 1 signaling pathway to suppress oxidative stress and inflammation(2023)
作者:Guangyao Fang, Xiuchuan Li, Fengyuan Yang, Ting Huang, Chenming Qiu, Ke Peng, Yongjian Yang, Cong Lan
期刊:PHYTOTHERAPY RESEARCH
DOI:10.1002/ptr.7991
影响因子:7.2
引用产品: MCF7 [MCF-7] 细胞, T-47D 细胞
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Q1:T-47D细胞不加胰岛素的话会影响细胞贴壁吗?
胰岛素不添加,细胞生长和增殖可能会受到影响,间接影响到细胞的贴壁性。建议细胞培养初期使用官网推荐的培养体系,细胞量多了保种后,再根据实验需求进行测试。
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Q2:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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