货号： CL-0412
Price： ￥1600 ￥1600
生长培养基： RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | PC-12 (Undifferentiated)（大鼠肾上腺嗜铬细胞瘤细胞（未分化）） |
| 生长特性 | 疏松贴壁 |
| 细胞形态 | 圆形 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 |
|
参考资料(来源文献)
| 背景描述 | PC-12(Undifferentiated)细胞是来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。PC-12(Undifferentiated)细胞表达神经生长因子（NGF）受体，NGF可诱导产生神经表型。PC-12(Undifferentiated)细胞不合成肾上腺素。 |
| 年龄（性别） | Male |
| 组织来源 | 肾上腺嗜铬细胞瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 嗜铬细胞瘤细胞 |
STR位点信息
STR鉴定图
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Calycosin ameliorates spinal cord injury by targeting Hsp90 to inhibit oxidative stress and apoptosis of nerve cells(2022)
作者：Mingdong Li, Hasiqiqige, Yanqiang Huan, Xiaolei Wang, Mingkai Tao, Tianqi Jiang, Hongbin Xie, Wu Jisiguleng, Wei Xing, Zhibo Zhu, Aitao Wang, Yongxiong He
期刊：JOURNAL OF CHEMICAL NEUROANATOMY
DOI：10.1016/j.jchemneu.2022.102190
影响因子：3.1
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miR?222-3p reduces neuronal cell apoptosis and alleviates spinal cord injury by inhibiting Bbc3 and Bim(2022)
作者：Qiangqiang Zhang, Gang Li, Jundong Kong, Junyu Dai, Zhongkai Fan, Jian Li
期刊：NEUROSCIENCE RESEARCH
DOI：10.1016/j.neures.2022.10.008
影响因子：2.9
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Fabrication and detection of a novel hybrid conductive scaffold based on alginate/gelatin/carboxylated carbon nanotubes (Alg/Gel/mMWCNTs) for neural tissue engineering(2022)
作者：Hailin Ma, Kai Yu, Hao Wang, Jiaqi Liu, Yuen Yee Cheng, Yue Kang, Hong Wang, Jingying Zhang, Kedong Song
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2022.101995
影响因子：2.6
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Atractylenolide III induces apoptosis by regulating the Bax/Bcl-2 signaling pathway in human colorectal cancer HCT-116 Cells in vitro and in vivo(2022)
作者：Dan Zhang, Xiaofang Li, Daqiang Song
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001136
影响因子：2.3
引用产品： HCT 116 细胞, A549 [A-549] 细胞, Hep G2 细胞, PC-12 (Undifferentiated) 细胞
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Macroscopic Supramolecular Assembly Strategy to Construct 3D Biocompatible Microenvironments with Site-Selective Cell Adhesion(2021)
作者：Changyu Wang, Cuiling Lin, Rui Ming
期刊：ACS Applied Materials & Interfaces
影响因子：9.5
引用产品： MG-63 细胞, PC-12 (Undifferentiated) 细胞, 重组人胰岛素溶液 (10mg/mL), MEM非必需氨基酸溶液 (NEAA)，100×, PUMC-HUVEC-T1, CCC-SMC-1 细胞
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Protective effect of plastrum testudinis extract on dopaminergic neurons in a Parkinsons disease model through DNMT1 nuclear translocation and SNCAs methylation(2021)
作者：Sen Ye, Jun Zhong, Jiapei Huang
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2021.111832
影响因子：7.5
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Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in H2O2-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling Pathways(2021)
作者：Qing Zhang, Ruo-Lan Li, Ting Tao, Jia-Yi Sun, Jia Liu, Ting Zhang, Wei Peng, Chun-Jie Wu
期刊：Oxidative Medicine and Cellular Longevity
影响因子：6.5
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Neuroprotective effects of NDEELNK from sea cucumber ovum against scopolamine-induced PC12 cell damage through enhancing energy metabolism and upregulation of the PKA/BDNF/NGF signaling pathway(2021)
作者：Yue Zhao, Yifei Dong, Qi Ge
期刊：Food & Function
影响因子：6.1
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A novel nonapeptide SSDAFFPFR from Antarctic krill exerts a protective effect on PC12?cells through the BCL-XL/Bax/Caspase-3/p53 signaling pathway(2021)
作者：Jingru Zheng, Mengqi Li, Chuanzhi Wang
期刊：Food Bioscience
DOI：10.1016/j.fbio.2021.101345
影响因子：5.2
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Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway(2021)
作者：Yong Xie, Yangping Chen, Yuyuan Zhu
期刊：Stem Cells International
影响因子：4.3
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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