PC-12 (Undifferentiated)(大鼠肾上腺嗜铬细胞瘤细胞(未分化))

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货号: CL-0412

Price: ¥1600 ¥1600

Size:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

生长培养基: RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120]

产品信息

产品名称 PC-12 (Undifferentiated)(大鼠肾上腺嗜铬细胞瘤细胞(未分化))
生长特性 疏松贴壁
细胞形态 圆形
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120]
培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃
培养方案B(可选) 生长培养基:
培养条件: 气相:, 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3次/周
注意事项
  1. 1.PC-12(Undifferentiated)细胞正常生长状态为悬浮聚团,若需要用NGF诱导时,需要使用多聚-L-赖氨酸溶液(货号PB180523)包被培养瓶后促使细胞贴壁;2.Undifferentiated、Low differentiation和High differentiation的区别:Undifferentiated的PC-12从形态上看是圆形的,聚团生长的漂浮细胞;Low differentiation的成多角形,有较短的突起;High differentiation的细胞有多个突起,突起数目不等,突触较长,类似神经元轴突。3.Low differentiation和High differentiation是在Undifferentiated的PC-12基础上诱导产生的,Low differentiation和High differentiation指的与神经细胞表型的相似程度,High differentiation更接近神经细胞表型。

参考资料(来源文献)

背景描述 PC-12(Undifferentiated)细胞是来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。PC-12(Undifferentiated)细胞表达神经生长因子(NGF)受体,NGF可诱导产生神经表型。PC-12(Undifferentiated)细胞不合成肾上腺素。
年龄(性别) Male
组织来源 肾上腺嗜铬细胞瘤
细胞类型 肿瘤细胞
肿瘤类型 嗜铬细胞瘤细胞

STR位点信息

STR鉴定图

  • Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber-Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair(2022)

    作者:Binghui Jin, Yiling Yu, Chenghao Lou, Xiaodi Zhang, Bowen Gong, Jinghao Chen, Xiangxiang Chen, Zihan Zhou, Liqun Zhang, Jian Xiao, Jiajia Xue

    期刊:Advanced Science

    DOI:10.1002/advs.202203296

    影响因子:17.5

    引用产品: PC-12 (Undifferentiated) 细胞, 多聚-L-赖氨酸溶液 (10×), PC-12 (Undifferentiated)细胞专用培养基

  • Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice(2022)

    作者:Libin Ni, Jian Xiao, Di Zhang

    期刊:Cell Death & Disease

    DOI:10.1038/s41419-022-04592-4

    影响因子:9.0

    引用产品: BV2 细胞, PC-12 (Undifferentiated) 细胞

  • Antidepressant-like effects of geniposide in chronic unpredictable mild stress-induced mice by regulating the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 networks(2022)

    作者:Yu Zhao, Qian Zhang, Yuzhu Yan, Xinbo Wang, Yin Shao, Cheng Mei, Tianyu Zou

    期刊:PHYTOTHERAPY RESEARCH

    DOI:10.1002/ptr.7702

    影响因子:6.4

    引用产品: PC-12 (Undifferentiated) 细胞

  • Alpiniae oxyphyllae fructus possesses neuroprotective effects on H2O2 stimulated PC12 cells via regulation of the PI3K/Akt signaling Pathway(2022)

    作者:Ruolan Li, Lingyu Wang, Qing Zhang, Huxinyue Duan, Die Qian, Fei Yang, Jun Xia

    期刊:Frontiers in Pharmacology

    DOI:10.3389/fphar.2022.966348

    影响因子:6.0

    引用产品: PC-12 (Low differentiation) 细胞

  • Taxifolin attenuates neuroinflammation and microglial pyroptosis via the PI3K/Akt signaling pathway after spinal cord injury(2022)

    作者:Zhenxin Hu, Lina Xuan, Tingting Wu, Nizhou Jiang, Xiangjun Liu, Jiazhen Chang, Te Wang, Nan Han, Xiliang Tian

    期刊:INTERNATIONAL IMMUNOPHARMACOLOGY

    DOI:10.1016/j.intimp.2022.109616

    影响因子:5.7

    引用产品: BV2 细胞, PC-12 (Undifferentiated) 细胞

  • A Novel Based-Network Strategy to Identify Phytochemicals from Radix Salviae Miltiorrhizae (Danshen) for Treating Alzheimer’s Disease(2022)

    作者:Bo Li, Yu-Rui Wu, Lan Li, Yu Liu, Zhu-Yun Yan

    期刊:MOLECULES

    DOI:10.3390/molecules27144463

    影响因子:4.9

    引用产品: PC-12 (Low differentiation) 细胞

  • The SWGEDWGEIW from Soybean Peptides Reduce Oxidative Damage-Mediated Apoptosis in PC-12 Cells by Activating SIRT3/FOXO3a Signaling Pathway(2022)

    作者:Guofu Yi, Mengyue Zhou, Qingfei Du, Shuibing Yang, Yuxia Zhu, Yining Dong, Yang Liu, He Li, You Li, Xinqi Liu

    期刊:MOLECULES

    DOI:10.3390/molecules27217610

    影响因子:4.9

    引用产品: PC-12 (Undifferentiated) 细胞

  • Biogenic Selenium Nanoparticles Attenuate Aβ25–35-Induced Toxicity in PC12 Cells via Akt/CREB/BDNF Signaling Pathway(2022)

    作者:Qiao Lei, Chen Yue, Dou Xina, Song Xiaofan, Xu Chunlan

    期刊:NEUROTOXICITY RESEARCH

    DOI:10.1007/s12640-022-00590-8

    影响因子:4.0

    引用产品: PC-12 (Low differentiation) 细胞

  • Protective effects of Dendrobium huoshanense polysaccharide on D-gal induced PC12 cells and aging mice, in vitro and in vivo studies(2022)

    作者:Mengjuan Ye, Junlin Liu, Guanghui Deng, Xiao Cai, Xiaoqian Zhang, Liang Yao, Jing Wu, Xianglin He, Daiyin Peng, Nianjun Yu

    期刊:JOURNAL OF FOOD BIOCHEMISTRY

    DOI:10.1111/jfbc.14496

    影响因子:3.7

    引用产品: PC-12 (Low differentiation) 细胞

  • Purification, Identification and Neuroprotective Effects of Proteins from Bombyx batryticatus in Glu-Stimulated PC12 Cells(2022)

    作者:Mei-Bian Hu, Xiang-Long Meng, Pu Wang, Shuo-Sheng Zhang, Chun-Jie Wu, Yu-Jie Liu

    期刊:Separations

    DOI:10.3390/separations9090236

    影响因子:3.3

    引用产品: PC-12 (Low differentiation) 细胞

  • Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?

    部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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