PC-12 (Undifferentiated)(大鼠肾上腺嗜铬细胞瘤细胞(未分化))

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货号: CL-0412

Price: ¥1600 ¥1600

Size:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

生长培养基: RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120]

产品信息

产品名称 PC-12 (Undifferentiated)(大鼠肾上腺嗜铬细胞瘤细胞(未分化))
生长特性 疏松贴壁
细胞形态 圆形
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[PM150110]+15% HS[164215]+5% FBS[164210]+1% P/S[PB180120]
培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃
培养方案B(可选) 生长培养基:
培养条件: 气相:, 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3次/周
注意事项
  1. 1.PC-12(Undifferentiated)细胞正常生长状态为悬浮聚团,若需要用NGF诱导时,需要使用多聚-L-赖氨酸溶液(货号PB180523)包被培养瓶后促使细胞贴壁;2.Undifferentiated、Low differentiation和High differentiation的区别:Undifferentiated的PC-12从形态上看是圆形的,聚团生长的漂浮细胞;Low differentiation的成多角形,有较短的突起;High differentiation的细胞有多个突起,突起数目不等,突触较长,类似神经元轴突。3.Low differentiation和High differentiation是在Undifferentiated的PC-12基础上诱导产生的,Low differentiation和High differentiation指的与神经细胞表型的相似程度,High differentiation更接近神经细胞表型。

参考资料(来源文献)

背景描述 PC-12(Undifferentiated)细胞是来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。PC-12(Undifferentiated)细胞表达神经生长因子(NGF)受体,NGF可诱导产生神经表型。PC-12(Undifferentiated)细胞不合成肾上腺素。
年龄(性别) Male
组织来源 肾上腺嗜铬细胞瘤
细胞类型 肿瘤细胞
肿瘤类型 嗜铬细胞瘤细胞

STR位点信息

STR鉴定图

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    作者:Ying Kong, Shulin Li, Miao Zhang

    期刊:Journal of Molecular Neuroscience

    DOI:10.1007/s12031-020-01770-x

    影响因子:3.1

    引用产品: PC-12 (Undifferentiated) 细胞

  • Preparation, structural characterization and neuroprotective effects of polysaccharides from the pericarp of Zanthoxylum bungeanum Maxim against H2O2-induced oxidative damage in PC12 cells(2021)

    作者:Lin Chen, Mei-Bian Hu, Zhi-Yang Chen, Gang Wang, Qing Su, Yu-Jie Liu

    期刊:SOUTH AFRICAN JOURNAL OF BOTANY

    DOI:10.1016/j.sajb.2021.06.026

    影响因子:2.3

    引用产品: PC-12 (Low differentiation) 细胞

  • Four new resorcinol derivatives with neuroprotective activities from Xylaria nigripes(2021)

    作者:Lanqing Li, Jing Li, Hongping Long

    期刊:Natural Product Research

    DOI:10.1080/14786419.2021.1897591

    影响因子:2.2

    引用产品: PC-12 (Undifferentiated) 细胞

  • microRNA-184 enhances the sensitivity of pheochromocytoma-12 cells to doxorubicin by targeting ADAM22(2021)

    作者:Nairui Zhao, Na Su, Guangya Wang

    期刊:Molecular & Cellular Toxicology

    DOI:10.1007/s13273-021-00116-x

    影响因子:1.7

    引用产品: PC-12 (Undifferentiated) 细胞

  • A value-added application of eugenol as acaricidal agent: The mechanism of action and the safety evaluation(2020)

    作者:Xiaofei Shang, Lixia Dai, Chenjie Yang

    期刊:Journal of Advanced Research

    DOI:10.1016/j.jare.2020.12.010

    影响因子:10.7

    引用产品: PC-12 (Undifferentiated) 细胞

  • Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes(2020)

    作者:Chengkang He, Peng Gao, Yuanting Cui

    期刊:Clinical and Translational Medicine

    DOI:10.1002/ctm2.205

    影响因子:10.6

    引用产品: PC-12 (Undifferentiated) 细胞

  • Neuroprotective Function of a Novel Hexapeptide QMDDQ from Shrimp via Activation of the PKA/CREB/BNDF Signaling Pathway and Its Structure–Activity Relationship(2020)

    作者:Dan Wu, Shuyu Zhang, Na Sun

    期刊:Journal Of Agricultural And Food Chemistry

    DOI:10.1021/acs.jafc.0c02689

    影响因子:6.1

    引用产品: PC-12 (Undifferentiated) 细胞

  • Optimal route of gold nanoclusters administration in mice targeting Parkinson’s disease(2020)

    作者:Jinqi Hu, Guanbin Gao, Meng He

    期刊:Nanomedicine

    DOI:10.2217/nnm-2019-0268

    影响因子:5.5

    引用产品: PC-12 (Undifferentiated) 细胞

  • Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway(2020)

    作者:Ruo-Lan Li, Qing Zhang, Jia Liu, Jia-yi Sun, Li-Ying He, Hu-Xinyue Duan, Wei Peng, Chun-Jie Wu

    期刊:Oxidative Medicine and Cellular Longevity

    DOI:10.1155/2020/3481758

    影响因子:5.1

    引用产品: PC-12 (Low differentiation) 细胞

  • The Effect of Protein-Rich Extract from Bombyx Batryticatus against Glutamate-Damaged PC12 Cells Via Regulating γ-Aminobutyric Acid Signaling Pathway(2020)

    作者:Li-Ying He, Mei-Bian Hu, Ruo-Lan Li, Rong Zhao, Lin-Hong Fan, Li Wang, Wei Peng, Yu-Jie Liu, Chun-Jie Wu

    期刊:MOLECULES

    DOI:10.3390/molecules25030553

    影响因子:3.3

    引用产品: PC-12 (Low differentiation) 细胞

  • Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?

    部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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