DMEM高糖
货号： PM150210
Price： ￥58
产品信息
| 产品简介 | Dulbecco's 改良培养基--DMEM（Dulbecco's Modified Eagle Medium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L（低糖型），后来又发展出葡萄糖含量为4500mg/L（高糖型），现已广泛应用于各种细胞的培养。 DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。 本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。 |
| 产品浓度 | 1× |
| 产品规格 | 500 mL |
| PH | 7.2-7.4 |
| D-葡萄糖 | 4500mg/L |
| HEPES | 无 |
| L-谷氨酰胺 | 4mM |
| NaHCO3 | 3700mg/L |
| 酚红 | 15mg/L |
| 丙酮酸钠 | 1 mM |
| 内毒素含量(EU/mL) | ＜3 |
| 储存条件 | 2-8℃，避光储存 |
| 运输条件 | 常温 |
| 注意事项 |
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Regulation of tumorigenesis and ferroptosis in non-small cell lung cancer by a novel BBOX1-AS1/miR-326/PROM2 axis(2023)
期刊：MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI：10.1007/s11010-023-04837-6
影响因子：4.300
引用产品： BEAS-2B Cells Line, A549 [A-549] Cells Line, SK-MES-1 Cells Line, NCI-H1299 Cells Line, NCI-H520 Cells Line, Ham's F-12K, MEM(with NEAA), RPMI-1640, DMEM(High glucose)
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Mitophagy contributes to zinc-induced ferroptosis in porcine testis cells(2023)
期刊：FOOD AND CHEMICAL TOXICOLOGY
影响因子：4.300
引用产品： ST Cells Line, DMEM(High glucose)
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Soluble thrombomodulin alleviates Diquat-induced acute kidney injury by inhibiting the HMGB1/IκBα/NF-κB signalling pathway(2023)
期刊：FOOD AND CHEMICAL TOXICOLOGY
影响因子：4.300
引用产品： DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
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Downregulation of the long noncoding RNA DSCR9 (Down syndrome critical region 9) delays breast cancer progression by modulating microRNA-504-5p-dependent G protein-coupled receptor 65(2023)
期刊：Human Cell
DOI：10.1007/s13577-023-00916-4
影响因子：4.300
引用产品： MCF 10A Cells Line, DMEM/F12, with L-alanyl-L-glutamine, HEPES, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, DMEM(High glucose), Fetal Bovine Serum, RPMI-1640, 293T [HEK-293T] Cells Line
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Inhibition of Mitochondrial Fission Alleviates Zearalenone-Induced Mitochondria-Associated Endoplasmic Reticulum Membrane Dysfunction in Piglet Sertoli Cells(2023)
期刊：Toxins
影响因子：4.200
引用产品： DMEM(High glucose)
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GREB1L overexpression is associated with good clinical outcomes in breast cancer(2023)
期刊：EUROPEAN JOURNAL OF MEDICAL RESEARCH
DOI：10.1186/s40001-023-01483-y
影响因子：4.200
引用产品： DMEM/F12 Medium, Leibovitz's L-15 Medium, MCF 10A Cell Complete Medium, DMEM (High glucose) Medium, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×
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Garbractin A, a Polycyclic Polyprenylated Acylphloroglucinol with a 4,11-dioxatricyclo[4.4.2.01,5]Dodecane Skeleton from Garcinia bracteata Fruits(2023)
期刊：ACS Omega
影响因子：4.100
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Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo(2023)
期刊：ACS Omega
影响因子：4.100
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Identification and preparation of selenium-containing peptides from selenium-enriched Pleurotus eryngii and their protective effect on lead-induced oxidative damage in NCTC1469 hepatocytes(2023)
期刊：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
影响因子：4.100
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High quantum yield nitrogen-doped carbon quantum dots: Green synthesis and application as “on-off” fluorescent sensors for specific Fe3+ ions detection and cell imaging(2023)
期刊：DIAMOND AND RELATED MATERIALS
DOI：10.1016/j.diamond.2023.109702
影响因子：4.100
引用产品： 5 mg/mL MTT solution, Hep G2 Cells Line, Fetal Bovine Serum, DMEM(High glucose)
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Q1:请问DMEM培养基中成分表没有写含有甲硫氨酸，是不含有这个成分吗？
是含有的，蛋氨酸就是甲硫氨酸。
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Q2:DMEM培养基放-20℃影响使用吗？
如果细胞培养基偶然被冻，自然溶解培养基后观察是否有沉淀产生；如果没有沉淀产生，培养基则可以正常使用；如果出现沉淀，意味着有部分营养成分析出，为了不影响细胞生长，补建议继续使用。
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