DMEM高糖
货号： PM150210
Price： ￥58
产品信息
| 产品简介 | Dulbecco's 改良培养基--DMEM（Dulbecco's Modified Eagle Medium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L（低糖型），后来又发展出葡萄糖含量为4500mg/L（高糖型），现已广泛应用于各种细胞的培养。 DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。 本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。 |
| 产品浓度 | 1× |
| 产品规格 | 500 mL |
| PH | 7.2-7.4 |
| D-葡萄糖 | 4500mg/L |
| HEPES | 无 |
| L-谷氨酰胺 | 4mM |
| NaHCO3 | 3700mg/L |
| 酚红 | 15mg/L |
| 丙酮酸钠 | 1 mM |
| 内毒素含量(EU/mL) | ＜3 |
| 储存条件 | 2-8℃，避光储存 |
| 运输条件 | 常温 |
| 注意事项 |
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GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline(2024)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2024.135101
影响因子：12.200
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Chemoproteomic profiling by bioorthogonal probes to reveal the novel targets of acrylamide in microglia(2024)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2024.136760
影响因子：12.200
引用产品： Fetal Bovine Serum, DMEM (High glucose) Medium
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A multi-omics approach reveals that lotus root polysaccharide iron ameliorates iron deficiency-induced testicular damage by activating PPARγ to promote steroid hormone synthesis(2024)
期刊：Journal of Advanced Research
DOI：10.1016/j.jare.2024.09.022
影响因子：11.400
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S1PR1 suppresses lung adenocarcinoma progression through p-STAT1/miR-30c-5 p/FOXA1 pathway(2024)
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-024-03230-5
影响因子：11.400
引用产品： DMEM (High glucose) Medium, 293T [HEK-293T] Cells Line, BEAS-2B Cells Line, NCI-H838 Cells Line, Fetal Bovine Serum, A549 [A-549] Cells Line, RPMI-1640 Medium, NCI-H1975 Cells Line
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Akt-FoxO signaling drives co-adaptation to insecticide and host plant stresses in an herbivorous insect(2024)
期刊：Journal of Advanced Research
DOI：10.1016/j.jare.2024.11.006
影响因子：11.400
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Hippo-YAP/TAZ-ROS signaling axis regulates metaflammation induced by SelenoM deficiency in high-fat diet-derived obesity(2024)
期刊：Journal of Advanced Research
DOI：10.1016/j.jare.2024.06.005
影响因子：11.400
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SNAI1 promotes epithelial-mesenchymal transition and maintains cancer stem cell-like properties in thymic epithelial tumors through the PIK3R2/p-EphA2 Axis(2024)
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-024-03243-0
影响因子：11.400
引用产品： RPMI-1640 Medium, Fetal Bovine Serum, DMEM (High glucose) Medium
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EIF4A3-mediated biogenesis of circSTX6 promotes bladder cancer metastasis and cisplatin resistance(2024)
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-023-02932-6
影响因子：11.300
引用产品： DMEM/F12, Ham's F-12K, RPMI-1640, DMEM(High glucose), Penicillin-Streptomycin Solution, 100×
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A novel red fluorescent and dynamic nanocomposite hydrogel based on chitosan and alginate doped with inclusion complex of carbon dots(2024)
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2024.122203
影响因子：11.200
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NORAD exacerbates metabolic dysfunction-associated steatotic liver disease development via the miR-511-3p/Rock2 axis and inhibits ubiquitin-mediated degradation of ROCK2(2024)
期刊：METABOLISM-CLINICAL AND EXPERIMENTAL
DOI：10.1016/j.metabol.2024.156111
影响因子：10.800
引用产品： DMEM (High glucose) Medium, Human Pancreatic Islet β Cells
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Q1:请问DMEM培养基中成分表没有写含有甲硫氨酸，是不含有这个成分吗？
是含有的，蛋氨酸就是甲硫氨酸。
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Q2:DMEM培养基放-20℃影响使用吗？
如果细胞培养基偶然被冻，自然溶解培养基后观察是否有沉淀产生；如果没有沉淀产生，培养基则可以正常使用；如果出现沉淀，意味着有部分营养成分析出，为了不影响细胞生长，补建议继续使用。
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