货号： CL-0602
Price： ￥1600 ￥1600
生长培养基： DMEM/F12[PM150312]+10% FBS[164210]+0.5% ITS-G（100×）[PB180429]+40 ng/mL Dexamethasone+1% P/S[PB180120]
产品信息
| 产品名称 | AML12（小鼠正常肝细胞） |
| 别称 | AML-12; AML 12; Alpha Mouse Liver 12 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:DMEM/F12[PM150312]+10% FBS[164210]+0.5% ITS-G（100×）[PB180429]+40 ng/mL Dexamethasone+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. |
| 年龄（性别） | 3M Male |
| 组织来源 | 肝脏 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | NO |
STR位点信息
STR鉴定图
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Pentagalloylglucose alleviates acetaminophen-induced acute liver injury by modulating inflammation via cGAS-STING pathway(2024)
作者：Zheng Congyang, Chen Yuanyuan, He Tingting, Xiu Ye, Dong Xu, Wang Xianling, Wen Xinru, Li Chengwei, Yao Qing, Chen Simin, Zhan Xiaoyan, Gao Lili, Bai Zhaofang
期刊：MOLECULAR MEDICINE
DOI：10.1186/s10020-024-00924-6
影响因子：6.0
引用产品： THP-1 细胞, 293 [HEK-293] 细胞, 293T [HEK-293T] 细胞, AML12 细胞
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Sesamin ameliorates nonalcoholic hepatic steatosis by inhibiting CD36-mediated hepatocyte lipid accumulation in vitro and in vivo(2024)
作者：Ya-ping Bai, Teng Zhang, Zheng-yan Hu, Yan Zhang, De-guo Wang, Meng-yun Zhou, Ying Zhang, Fang Zhang, Xiang Kong
期刊：BIOCHEMICAL PHARMACOLOGY
影响因子：5.8
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Identification and validation of cuproptosis-related genes in acetaminophen-induced liver injury using bioinformatics analysis and machine learning(2024)
作者：Guo Zhenya, Liu Jiaping, Liang Guozhi, Liang Haifeng, Zhong Mingbei, Tomlinson Stephen, He Songqing, Ouyang Guoqing, Yuan Guandou
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2024.1371446
影响因子：5.7
引用产品： AML12细胞专用培养基, AML12 细胞
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Identification of potential pathogenic hepatic super-enhancers regulatory network in high-fat diet induced hyperlipidemia(2024)
作者：Yingying Hu, Run Xu, Jing Feng, Qingwei Zhang, Lifu Zhang, Yiyang Li, Xiuxiu Sun, Jin Gao, Ximing Chen, Menghan Du, Zhouxiu Chen, Xin Liu, Yuhua Fan, Yong Zhang
期刊：JOURNAL OF NUTRITIONAL BIOCHEMISTRY
DOI：10.1016/j.jnutbio.2024.109584
影响因子：5.6
引用产品： AML12 细胞, AML-12细胞专用培养基
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Qizhu Anticancer Prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes(2024)
作者：Rui Hu, Jing Li, Qi Huang, Xin Zhong, Jialing Sun, Jinyu Yi, Lanfen Peng, Xinning Liu, Yuan Yang, Wenmin Yang, Yan Wang, Wenfeng Ma, Wenxing Feng, Youhua Xu, Xiaozhou Zhou
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子：5.4
引用产品： 特级胎牛血清, AML12 细胞, AML12细胞专用培养基
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GRK2 mediates cisplatin-induced acute liver injury via the modulation of NOX4(2024)
作者：Wang Qianlei, Li Mengyang, Duan Fei, Xiao Kangjun, Sun Qing Qing, Cheng Jiang rui, Ni Lei, Xu Zhengkun, Xu Bingfa, Xiao Feng, Kuai Jiajie, Wei Wei, Wang Chun
期刊：CELL BIOLOGY AND TOXICOLOGY
DOI：10.1007/s10565-024-09940-y
影响因子：5.3
引用产品： AML12 细胞
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Triptolide induces hepatotoxicity by promoting ferroptosis through Nrf2 degradation(2024)
作者：Guo Lin, Yang Yan, Ma Jiating, Xiao Mingxuan, Cao Rong, Xi Yang, Li Tao, Huang Tianlong, Yan Miao
期刊：CELL BIOLOGY AND TOXICOLOGY
DOI：10.1007/s10565-024-09930-0
影响因子：5.3
引用产品： AML12 细胞
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Digoxigenin activates autophagy in hepatocellular carcinoma cells by regulating the PI3K/AKT/mTOR pathway(2024)
作者：Ma Mengqing, Hu Rui, Huang Qi, Li Jing, Lv Minling, Sun Jialing, Zhong Xin, Yi Jinyu, Peng Lanfen, Feng Wenxing, Ma Wenfeng, Han Zhiyi, Zhang Wei, Sun Xinfeng, Zhan Bolin, Liu Xingning, Zhou Xiaozhou
期刊：Cancer Cell International
DOI：10.1186/s12935-024-03602-z
影响因子：5.3
引用产品： AML12 细胞, Hep G2 细胞, AML12细胞专用培养基
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Identification and validation of cuproptosis-related molecular clusters in non-alcoholic fatty liver disease(2024)
作者：Changxu Liu, Zhihao Fang, Kai Yang, Yanchao Ji, Xiaoxiao Yu, ZiHao Guo, Zhichao Dong, Tong Zhu, Chang Liu
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子：5.3
引用产品： AML12 细胞
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10-Hydroxy-2-decenoic acid attenuates nonalcoholic fatty liver disease by activating AMPK-α signaling pathway(2024)
作者：Minyi Tang, Xinzi Wang, Shuai Wang, Chaofeng Xing, Qihua Xu, Yunping Mu, Xiaoli Wu, Allan Zijian Zhao, Fanghong Li
期刊：BIOCHEMICAL PHARMACOLOGY
影响因子：5.3
引用产品： AML12 细胞
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Q1:AML 12(小鼠正常肝细胞)DMEM/F12＋10%FBS＋0.5%ITS-G＋40ng/mlDexamethasone＋1%P/S这个加ITS-G0.5%就可以呀，我看您这个产品是100x的，那500ml体系里面是加2.5ml就行对吗？
是的，500mL培养基里加ITS-G（PB180429）2.5ml就可以。
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Q2:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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